Registration Number
0224U033313,
(0124U003464)
, R & D reports
Title
Molecular mechanisms of the therapeutic effect of the transplantation of placental mesenchymal stromal cells in the treatment of mine-explosive injuries of the lower limbs in humans
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Лікування мінно-вибухових травм нижніх кінцівок із застосуванням множинних ін’єкцій ПМСК та оцінка зімни в профілі експресії генів в тканинах рани та мононуклеарах периферійної крові людини
Head
Shablii Volodymyr A., Кандидат біологічних наук
Registration Date
24-12-2024
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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The goal of the project is to clarify the molecular mechanisms of the therapeutic effect of transplantation of human placental mesenchymal stromal cells (PMSCs) on the healing of landmine-explosive limb wounds in complex surgical treatment.
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As a result of the implementation of stage No. 1 of the R&D, studies were conducted. As a result of the first (intermediate) stage of the project, biopsy material and peripheral blood were collected from patients with mine-blast wounds after injection of PMSC preparations and under standard treatment conditions. mRNA was isolated from biopsy material and blood cell samples for transcriptome analysis. Mononuclear cells were isolated from the peripheral blood of patients with mine-blast wounds and changes in immunophenotype were determined after their co-cultivation with PMSCs in vitro. The obtained mRNA samples will be used in the next stage to determine changes in gene expression on the surface of the mine-blast wound after injection of PMSC preparations.
Product Description
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Bukreieva Tetiana V.
Saliutin Ruslan V.
Symulyk Yevhen V.
Skrypkina Inessa Ya.
Ustymenko Alina M.
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2024-12-24
