Registration Number
0225U000263,
(0120U100381)
, R & D reports
Title
Cryopreservation of spermatogonial stem and accessory regulatory cells of the testes with using of biopolymer gels.
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Підбір оптимальних умов кріоконсервування сперматогоніальних стовбурових та акцесорно-регуляторних клітин сім’яників з використанням біополімерних гелів шляхом вітрифікації.
Head
Holtsev Anatolii M., Доктор медичних наук
Registration Date
08-01-2025
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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Experimental reasoning of the possibility of using biopolymer gels to improve the preservation of the morphofunctional state of spermatogonial stem and accessory-regulatory cells of the testes of rat at the stages of cryopreservation
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Now, the problem of infertility is an important component of modern medicine. The aim of the work was to determine the optimal cryopreservation conditions by vitrification of spermatogonial stem and accessory regulatory cells of the testes (SS and ARCs) using biopolymer gels. It was established that the optimal cryopreservation conditions by vitrification of SSTARC are the use of fibrin gel with the addition of 15% DMSO, 18% glycerol and 0.5M sucrose as a cryoprotective medium. These conditions ensure the preservation of membrane integrity, phenotype, metabolic activity, antioxidant system, proliferative activity and do not lead to the activation of apoptosis processes in SS and ARCs of rat testes. The results obtained can be used to develop effective cryopreservation protocols by vitrification of SSTARC and the creation of spermatogonial stem cells cryobanks in order to restore fertility in men.
Product Description
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Volkova Nataliia O.
Yukhta Mariia S.
Sokil Larysa V.
Stepaniuk Liudmyla V.
Chernyshenko Liudmyla H.
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2025-01-08
