Registration Number
0225U000396,
(0120U100381)
, R & D reports
Title
Cryopreservation of spermatogonial stem and accessory regulatory cells of the testes with using of biopolymer gels.
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Визначення композиційного складу середовища реабілітації для сперматогоніальних стовбурових та акцесорно-регуляторних клітин сім’яників після кріоконсервування з використанням біополімерних гелів.
Head
Holtsev Anatolii M., Доктор медичних наук
Registration Date
10-01-2025
Organization
Institute of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine
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Experimental reasoning of the possibility of using biopolymer gels to improve the preservation of the morphofunctional state of spermatogonial stem and accessory-regulatory cells of the testes of rat at the stages of cryopreservation
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The aim of the work was to determine the composition of the rehabilitation medium for spermatogonial stem and accessory regulatory cells of the testes after cryopreservation using biopolymer gels. It was found that the addition of a combination of carbon nanoparticles C60 (15 μg/ml) and bovine serum albumin (2%) to the rehabilitation medium increases the metabolic and antioxidant activity of cryopreserved spermatogonial stem and accessory regulatory cells of the testes of rats compared to control samples. It was noted that the combined use of carbon nanoparticles C60 and bovine serum albumin is more effective compared to their use separately. The results obtained can be used to develop effective protocols for cryopreservation of spermatogonial stem and accessory regulatory cells of the testes and the creation of spermatogonial cell cryobanks for the purpose of restoring fertility in men.
Product Description
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Yukhta Mariia S.
Sokil Larysa V.
Volkova Nataliia O.
Stepaniuk Liudmyla V.
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2025-01-10
