Registration Number
0225U000531,
(0123U101597)
, R & D reports
Title
Development of new approaches to the assessment of radiation-induced and radiation-inducible damage to the genome of human somatic cells.
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Встановлення рівня радіаційно-індукованих in vitro пошкоджень генома в лімфоцитах периферичної крові за цитогенетичними та молекулярно-генетичними показниками.
Head
Kurinnyi Denys A., Кандидат біологічних наук
Registration Date
13-01-2025
Organization
State Institution "National Research Center for Radiation Medicine, Hematology and Onkology of the National Academy of Medical Sciences of Ukraine"
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Development of a complex system for evaluating the target and non-target effects of ionizing radiation on the genome of peripheral blood lymphocytes to determine the individual radiosensitivity of a person.
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The object of the study is the metaphase plate and nuclear DNA of peripheral blood lymphocytes of 15 conditionally healthy individuals - volunteers.
The aim of the work is to investigate the relationship between the frequency and spectrum of chromosomal aberrations and the level of methylation, DNA damage and apoptosis in peripheral blood lymphocytes of conditionally healthy volunteers, irradiated in vitro at a dose of 1.0 Gy
Research methods - molecular genetic, cytogenetic, analytical, statistical.
In the study using a comprehensive approach, radiation-induced quantitative and structural changes in chromosomes were detected, and abnormalities in the genome were assessed by the level of methylation, the intensity of apoptosis, and the degree of DNA breaks. A significant increase in all indicators was established: aberrant metaphases with chromosomal aberrations, the frequency of which was (20.97 ± 0.89)%, versus (1.66 ± 0.35)% in the control, DNA breaks were recorded (13.48 ± 0.98)% versus (6.34 ± 0.72)% in the control, and the degree of apoptosis was 3.56 ± 0.71, versus 0.91 ± 0.33 in the control.
The conducted study, which characterizes the state of the human somatic cell genome at several levels of organization, an increased frequency of chromosomal aberrations with the presence of dicentric chromosomes, which is an indicator of radiation exposure, as well as changes in DNA methylation or gene expression after exposure to ionizing radiation, will make it possible to understand the mechanisms underlying these changes and obtain a more complete picture of individual radiosensitivity. The results obtained through an integrated approach will play a primary role in complex cases when it is necessary to confirm or refute the nature of the mutational damage caused by ionizing radiation.
Product Description
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Kurinnyi Denys A
Neumerzhytska Liubov V.
Romanenko Maryna H.
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2025-01-13
