Registration Number
0225U000857,
(0122U000374)
, R & D reports
Title
Study of the impact of native biostructures on microbial communities and creation of highly productive nutrient media based on them
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Вивчення впливу нативних біоструктур на мікробні спільноти та створення на їх основі високопродуктивних живильних середовищ
Head
Isajenko Olena Yu., Доктор медичних наук
Registration Date
20-01-2025
Organization
State Institution "I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine"
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Based on the use of native biologically active substances of the dominant and associative microflora of the respiratory tract to substantiate the conceptual provisions of the method of targeted changes in species and quantitative composition of microbial communities and develop highly productive nutrient media for cultivating demanding bacteria
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It has been established that certain ultrasonic disintegrates of microorganisms and their combinations can sustain the viability of other cultures of bacteria to varying degrees. It has been shown that individual and combined bacterial substrates can be used as growth factors for cultivating demanding microorganisms, replacing animal-derived products, in particular, serum from cattle blood. It has also been established that substrates with higher concentrations of combined biologically active substances have higher nutritional value. The effectiveness of adding bacterial disintegrates, both individually and in combinations, to nutrient agar has been demonstrated to enrich the latter with growth factors to support the growth of various species of highly demanding microorganisms, instead of blood, specifically: blood agar and yeast agar. Individual disintegrates were comparable to commercial yeast agar, while combinations of disintegrates outperformed yeast agar regarding microbial cell growth. There was likely a greater increase in the bacterial mass of microorganisms when disintegrates were applied in combination than when used separately. Additionally, the statistical nutritional value was higher for the combination of ultrasonic disintegrates compared to individual disintegrates. Individual disintegrates and combinations of disintegrates influence the biochemical parameters and pathogenicity factors of bacterial infection
Product Description
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Isaienko Olena Yu.
Bilozerskyi Volodymyr I.
Koliada Tetiana I.
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2025-01-20
