Registration Number
0225U000999,
(0123U101591)
, R & D reports
Title
Study of modification of radiation-induced and tumor-induced genome damage in peripheral blood lymphocytes of individuals with glioblastoma
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Дослідження зміни частоти і спектру аберацій хромосом та показників пошкодження ДНК і рівня апоптозу в лімфоцитах периферичної крові осіб з верифікованим діагнозом гліобластома під впливом іонізуючого випромінювання в дозі 1,0 Гр.
Head
Kurinnyi Denys A., Кандидат біологічних наук
Registration Date
22-01-2025
Organization
State Institution "National Research Center for Radiation Medicine, Hematology and Onkology of the National Academy of Medical Sciences of Ukraine"
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Determine the possibility of modification of tumor-induced and radiation-induced damage by astaxanthin in non-irradiated and irradiated in vitro lymphocytes of peripheral blood of persons diagnosed with glioblastoma
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The object of the study is peripheral blood lymphocytes of conditionally healthy individuals and glioblastoma patients
The purpose of the work is to study the effect of glioblastoma on changes in the frequency and spectrum of chromosome aberrations, indicators of DNA damage and the level of apoptosis in irradiated lymphocytes of peripheral blood of patients.
Research methods: method of comet electrophoresis of individual cells (Comet assay); cytogenetic.
The level of genomic instability in irradiated peripheral blood lymphocytes of 10 people (control group) and 20 glioblastoma patients (examination group) was compared.
Features of the modifying effect of glioma on the development of radiosensitivity in patients were revealed: after irradiation, in comparison with the control group, a statistically significant (p < 0.001) increase in the frequency of aberrant cells in peripheral blood lymphocytes of patients with glioblastoma was found, which indicates an increase in chromosomal instability under the influence of the tumor; under the joint influence of radiation and glioblastoma, a statistically significant increase in the frequency of cells in a state of apoptosis was recorded
It has been established that exposure to glioblastoma leads to an increase in the level of chromosomal aberrations and activates the process of apoptosis, which is a key mechanism for regulating the development of genomic instability in somatic untransformed cells of individuals with glioblastoma
Product Description
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Hlavatskyi Oleksandr Ya.
Demchenko Olena M.
Zemskova Oksana V.
Mekheda Liudmyla V.
Romanenko Maryna H.
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2025-01-22
