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Information × Registration Number 0225U001550, (0122U000894) , R & D reports Title Correction of metabolic syndrome by sulforaphane-enriched preparations of broccoli sprouts popup.stage_title Корекція метаболічного синдрому збагаченими сульфорафаном препаратами з проростків броколі Head Lushchak Volodymyr І., Доктор біологічних наук Registration Date 02-02-2025 Organization Vasyl Stefanyk Precarpathian National University, State Higher Educational Institution popup.description1 Based on broccoli seedlings, create a sulforaphane-enriched preparation that will reduce the symptoms of metabolic syndrome and associated inflammatory processes, as well as strengthen the defense system against reactive oxygen species and carbonyl compounds. popup.description2  During the project implementation, a reliable method for germinating broccoli seeds was developed and optimized to produce two plant preparations: sprouted seeds and green sprouts, in which the content of major biologically active compounds was partially determined. In particular, the project team developed an inexpensive quantitative method for determining the content of sulforaphane, the main bioactive compound in broccoli. Broccoli sprouted seeds and green sprouts obtained in the laboratory were tested on a model of metabolic syndrome in flies and mice. To induce metabolic syndrome, flies were fed a high-sucrose nutrient medium, and mice were fed a cafeteria diet with high-calorie cafeteria foods such as chocolate crackers, chocolate-coated nuts, and chocolate. To study the effect of the sprouted seeds and green sprouts, they were added to the nutrient medium of flies and the feed of mice, respectively. It was established that consuming the high-calorie nutrient medium increased the level of triacylglycerols (fat) in the body of flies and decelerated their development, while broccoli sprouted seeds did not prevent these effects. The cafeteria diet in mice promoted signs of metabolic syndrome, such as increased visceral fat mass, elevated blood glucose, and triacylglycerols (fats). Consuming broccoli sprouted seeds did not prevent visceral fat accumulation, but did reduce glucose and triacylglycerol levels. In addition, the cafeteria diet promoted moderate oxidative stress and signs of inflammation in the liver, and the addition of broccoli sprouted seeds partially reversed these effects and stimulated positive changes in gene transcription levels, indicating improved metabolic regulation and possible anti-inflammatory effects. Overall, the results confirm that broccoli sprouted seeds can have significant positive effects on metabolic health, particularly via the activation of antioxidant and anti-inflammatory mechanisms, while green sprouts did not have pronounc Product Description popup.authors Ivanochko Mar'ian V. Bailiak Mariia M. Dmytriv Tetiana R. Laba Olha I. Lylyk Mariia P. Lushchak Volodymyr I. Semchyshyn Halyna M. Shvadchak Volodymyr V. Shmihel Halyna V. popup.nrat_date 2025-02-02 Close