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Information × Registration Number 0225U002304, (0120U100849) , R & D reports Title Targeted genome changes and pleiotropic effects in genetically transformed plant systems popup.stage_title Молекулярно-біологічний аналіз нащадків отриманих трансгенних рослин, дослідження успадкування перенесених генів та пов'язаних з ними фенотипічних ознак. Head Kuchuk Mykola V., Доктор біологічних наук Registration Date 03-03-2025 Organization Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine popup.description1 The aim of scientific research is to purposefully change the genome of economically valuable plants of various species by the method of genetic transformation, to study phenotype changes, gene inheritance features and pleiotropic effects resulting from the expression of heterologous genes. popup.description2  During the reporting period, an analysis of biotechnological plants and transgenic root lines of various species was conducted to confirm the expression of transferred genes, transgene inheritance, and potential pleiotropic effects associated with transgenesis. The impact of heterologous expression of the colicin M antibacterial protein was studied in transgenic lettuce, mizuna, carrot, alfalfa, and kale plants. A pleiotropic effect of recombinant colicin M expression was observed in kale plants, which accumulated the protein. These plants exhibited less intense coloration of young leaves, along with reduced chlorophyll and flavonoid levels. However, hybridization of the transgenic kale line with the wild-type parental line resulted in a heterosis effect, producing hybrids with larger leaves and greater biomass. No pleiotropic effects were observed in other plant species accumulating recombinant colicin M. Additionally, no pleiotropic effects or phenotypic changes were detected in biotechnological rapeseed plants obtained through genetic transformation with the epsps gene, which confers glyphosate resistance. It was also established that methyl jasmonate (100 µM) inhibited root growth in all examined plant species (Artemisia tilesii, A. vulgaris, Cichorium intybus, and Calendula officinalis) by 1.3 to 3 times. However, it significantly stimulated flavonoid synthesis, and root extracts from plants cultivated in methyl jasmonate-enriched medium exhibited higher free radical scavenging activity, which correlated with the increased flavonoid content. Product Description popup.authors Bohdanovych Taisa A. Bulko Olha V. Vasylenko Maksym Yu. Duplii Volodymyr P. Lystvan Kateryna V. Luchakivska Yuliia S. Loshyna Liudmyla Н. Maistrov Petro D. Matvieieva Nadiia A. Makhorina Olha K. Nifantova Svitlana M. Ovcharenko Olha O. Ratushniak Yakiv I. Rudas Volodymyr А. Sindarovska Yana R. Symonenko Yurii V. Shakhovskyi Anatoli M. Shcherbаk Nаtаliia L. Yaroshko Olha M. popup.nrat_date 2025-03-03 Close