Registration Number
0225U002320,
(0121U113840)
, R & D reports
Title
Study of the lactobacilli, bifidobacteria and opportunistic pathogens of the human microbiota influence on the features of mechanisms realization of metabolic disturbances in the tumor process (2.2.5.447)
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Дослідження показників міграційної та інвазивної активності, a також туморогенності in vitro клітин РМЗ людини різного ступеня злоякісності під впливом бактерій родів Bifidobacterium, Lactobacillus, Staphylococcus і Pseudomonas
Head
Lykhova Oleksandra O., Кандидат біологічних наук
Chekhun Vasyl F., Доктор медичних наук
Registration Date
03-03-2025
Organization
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine
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To determine the role of opportunistic pathogens of the human microbiota, lactobacilli and bifidobacteria in the metabolic processes of breast cancer cells with different degree of malignancy
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The effect of saprophytic and opportunistic bacteria - human microbiota members - on the migratory and invasive activity of human breast cancer (ВС) cells in vitro was investigated. The results obtained indicate that lactic acid probiotic bacteria B. animalis and L. rhamnosus significantly inhibit the migratory and invasive activity of MCF-7 and MDA-MB-231 cells. In BC cells, when co-cultured with B. animalis, a significant increase in the expression of the signaling molecule STAT-6 and the glucose transporter GLUT-1 is observed. The increase in the expression of these proteins indicates a rise in the absorption and metabolism of glucose via the glycolytic pathway. It was shown that in BC cells characterized by a low degree of malignancy and in which a high insulin receptor (IR) expression is associated with a poor prognosis, bifidobacteria caused a decrease in the expression of IR, and in BC cells of medium and high degree of malignancy, on the contrary, an increase in the IR expression was noted, which may lead to a decrease in the insulin resistance of these cells. Studies of the complex effect of the B. subtilis metabolite IMV B-7724 and B. animalis on the antitumor immune response of mice with solid Ehrlich adenocarcinoma revealed that in animals of this group there is an increase in the functional activity of NK cells, cytotoxic T lymphocytes, and macrophages of the M1 and M2 types.
Product Description
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Bezdieniezhnykh Nataliia O.
Virych Petro A.
Verbinenko Natalia M.
Voeykova Iryna M.
Hetman Kateryna I.
Hohol Serhii V.
Zhuk Oleksandr M.
Kozak Tamara P.
Konovalenko Volodymyr F.
Konovalenko Serhii V.
Kukurudza Dmytro V.
Luk'ianova Yelyzaveta D.
Saulenko Kyrylo O.
Symchych Tetiana V.
Tarnopolska Kateryna D.
Tyshchenko Liudmyla M.
Todor Ihor M.
Fedorchenko Larysa V.
Fedosova Nataliia I.
Khaietskyi Ihor K.
Cheremshenko Nadiia L.
Chumak Alina V.
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2025-03-03
