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Information × Registration Number 0225U002323, (0121U113837) , R & D reports Title Investigation of the combined effect of radiation and chemotherapy on genetic and metabolic changes in peripheral blood lymphocytes of patients with cervical cancer (2.2.2.445) popup.stage_title Визначити можливість та особливості застосування радіопротектора для захисту немалігнізованих клітин хворих на РШМ від ускладнень за умов проведення променевої терапії Head Domina Emiliia A., Доктор біологічних наук Registration Date 03-03-2025 Organization R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology National Academy of Sciences of Ukraine popup.description1 Determine and substantiate the patterns of induction of genetic and metabolic changes in highly radiosensitive blood cells of patients with common forms of cervical cancer during combined radiation and chemotherapy and to develop approaches to selective protection of healthy cells of patients from toxicity and radiation complications popup.description2  For the first time, distinct effects of potential radioprotector melatonin (MT) on oxidative-reduction and genotoxic processes in blood of patients with cervical cancer (ССP) and healthy donors (HD) under in vitro ionizing radiation exposure were identified. Radiation of HD blood led to moderate increase in TMP, generation of ROS, significant number of DNA double strand breaks (DSB) (up to 1.7 times), and cells in early apoptosis stage (up to 2.4 times) in lymphocytes (PBL), as well as increase in lipid peroxidation (LPO) processes (up to 1.3 times) in plasma. A moderate increase in LPO and ROS generation was observed in CCP, while increase in DSB DNA and apoptosis in PBL was lower than in HD. MT administration decreased the enlarged TMP in PBL after irradiation of both HD and CCP blood. MT also reduced ROS generation and DNA damage (up to 1.4 times at a 6 Gy dose), apoptosis (up to 2.0 times), and plasma LPO in PBL of HD. In CCP, MT reduced increased content of apoptotic PBL (up to 1.3 times), but with combined radiation exposure, only a moderate drop in apoptosis was observed, while DSB DNA level reduced by up to 2.3 times and plasma LPO - up to 1.4 times. MT increased platelet survival in the CCP and HD blood by up to 1.9 times, both with its separate action and under radiation exposure. PBL survival of HD increased with MT and radiation exposure, whereas in CCP, it decreased both with MT alone and after radiation exposure (up to 1.6 times). Thus, obtained new data on MT modifying effect on radiosensitive blood cells in HD and primary CCP, depending on MT concentration and radiation dose. Radioprotective effect of MT during in vitro X-ray irradiation of blood was manifested by increase in platelet survival, a decrease in LPO intensity, ROS generation, DSB DNA formation, and early apoptosis-associated changes in HD. The MT decreased elevated level of spontaneous apoptosis in CCP, although it had little effect on level of radiation-induced early apoptosis. Product Description popup.authors Hlavin Oleksii A. Dumanskyi Yurii V. Kondrashova Halyna H. Makovetska Liudmyla I. Mykhailenko Viktor M. Prokopenko Ihor V. popup.nrat_date 2025-03-03 Close