Registration Number
0225U002537,
(0122U000745)
, R & D reports
Title
Determination of molecular genetic features of precancerous conditions of the cervix and endometrium, development of new approaches to assessing the risk of their progression and prevention of female genital cancer
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Проведення гістологічних, імуногістохімічних, імунофлюоресцентних досліджень. Визначення діагностичних та прогностичних критеріїв прогресії передракових станів.
Head
Potapov Valentyn O., д.мед.н.
Registration Date
18-03-2025
Organization
Dnipro State Medical University
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Reduce the risk of cervical and endometrial cancer in women with precancerous pathology of the cervix and endometrium by developing objective molecular cell markers for diagnosing and predicting the progression of carcinogenesis and timely early prevention and treatment.
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In morphological samples of endometrial hyperplasia without atypia (n 30) and with atypia (n 30), as well as endometrium without pathology (n 60), an immunohistochemical determination of intracellular antigens that initiate and control cell proliferation (cyclin Cdk-D1, gene p21) was carried out , Ki-67, ER, PGR, β-catenin, E-cadherin, MMP-9, BAC, Bcl-2, caspase-3, VEGF, eNOS, COX 2).
The molecular mechanism of initiation of hyperproliferation of the endometrium with the participation of estrogen hormones, ER, PGR, cyclin Cdk-D1, as well as the mechanism of suppressive effect on the proliferation of the p21 gene was determined. It was established that the expression of Ki-67 and cyclin Cdk D1 in endometrial glands without atypia and with atypia was significantly lower than in normal endometrium. The authors suggest that the molecular mechanism of endometrial hyperplasia is not related to an increase in the rate of endometrial cell mitoses, but to the accumulation of cells with incomplete cellular differentiation due to delayed menstruation, which had low expression values of the cell adhesion proteins E-cadherin and β-catenin.
Based on the obtained data, molecular characteristics of the phenotype of normal endometrium, endometrial hyperplasia without atypia and with cell atypia were determined. It has been proven that endometrial hyperplasia without atypia and with atypia have different molecular phenotypes, which indicates their different etiology and pathogenesis.
Molecular markers have been identified that can become criteria for predicting progression and the probability of carcinogenesis of endometrial hyperplasia without atypia, allowing to develop new differentiated approaches to the diagnosis and therapy of this pathology.
Product Description
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Islamova Svitlana K.
Harahulia Iryna S.
Demchenko Tetiana V.
Madii Liudmyla M.
Poslavska Oleksandra V.
Khaskhachykh Dmytro A.
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2025-03-18
