Registration Number
0225U002723,
(0122U200159)
, R & D reports
Title
Profiles and functional role of metabolites of bacteria of the genera Bacillus and Pseudomonas
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Прогнозування біологічної активності досліджуваних штамів бактерій роду Bacillus на основі їх метаболічних профілів методом хемоінформативного аналізу
Head
Avdieieva Liliia V., Доктор медичних наук
Registration Date
01-04-2025
Organization
Institute of Microbiology and Virology. D.K. Zabolotny National Academy of Sciences of Ukraine
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Based on the obtained metabolic profiles of bacteria of the genera Bacillus and Pseudomonas to determine the functional role of their metabolites
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It has been established that the quantitative and qualitative composition of metabolites of Bacillus bacteria significantly depends on the culture medium. Thus, when aerobic spore-forming microorganisms are grown on sugar-containing media, the main VOCs are butanediol and acetoin, and branched-chain fatty acids on peptone-containing media. It was found that the expression level of the gene encoding the synthesis of exogenous phytase in B. velezensis 20F and Bacillus sp.41 strains was 3-5 times higher than in the control strain. Thus, the manifestation of high exogenous phosphate-mobilising (phytase type) and antagonistic (lipopeptide antibiotic synthesis) activity of B. velezensis 20F and Bacillus sp.41 strains was confirmed, which can be recommended as a basis for biological products for plant production. It was found that the process of development of P. synxantha UCM B-399 under conditions of deep cultivation on rolling pin takes approximately 65-68 h, after which the culture enters the phase of dying. The active growth of the strain stops after 39-40 h with the transition of P. synxantha UKM B-399 to the stationary phase. It was also noted that at the end of the strain cultivation process, the colour of the culture fluid changed to yellow-orange, which may be associated with the synthesis of phenazine derivatives, including their oxidised forms, in particular oxyphenazines. It was found that P. aeruginosa strain UKM B-3 was characterised by more intensive biofilm formation during all periods of observation compared to P. aeruginosa strain UKM B-10. This indicates the presence of strain specificity of biofilm formation and the need to compare the results with the control values. Thus, the methodology for studying biofilm formation of Pseudomonas aeruginosa on 96-well plates was standardised.
Product Description
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Balko Oleksandr B.
Balko Olha I.
Boretska Kateryna Ye.
Hrabova Hanna Yu.
Direnko Dmytro I.
Drahovoz Ihor V.
Klochro Vitalii V.
Kozyreva Valentyna O
Korzh Yuliia V.
Korol Tsvitana O.
Livinska Olena P.
Mozhaieva Larysa L.
Okhota Olha A.
Poberezhna Liudmyla M.
Poliakova Alina V.
Rybalchenko Nataliia P.
Kharkhota Maksym A.
Kharchuk Maksym S.
Yaroshenko Liudmyla V.
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2025-04-01
