Registration Number
0225U002801,
(0124U001464)
, R & D reports
Title
The concept of developing innovative protein-based wound-healing materials
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Отримання білкових молекул та пептидів з різних джерел сировини та їхня загальна характеристика.
Head
Berehova Tetiana V., Доктор біологічних наук
Registration Date
04-04-2025
Organization
Taras Shevchenko National University of Kyiv
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The project aims to develop the concept of the creation of innovative wound-
healing agents (composites) based on protein molecules (proteolytic enzymes,
collagen, peptides), to investigate mechanisms and evaluate the effectiveness of
wound-healing agents on various wound models.
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The methodology for obtaining hydrolytic enzymes, collagen, and biologically active peptides was optimized, considering the specific characteristics of the raw materials and the need to obtain target molecules while preserving their maximum functional activity. The molecular weight of the obtained enzymes and peptides was assessed, and the substrate specificity of proteolytic enzymes was determined using a range of protein and chromogenic substrates. Their pH and temperature optima were also established. A qualitative and quantitative characterization of the obtained collagen was conducted, and its potential use as a basis for the development of collagen gels was evaluated. Additionally, the antioxidant and antimicrobial activities of the obtained peptide molecules were determined. Conditions and the most effective combinations of protein molecules and peptides were selected for the development of wound-healing agents in a formulation optimized for application to the wound surface, considering the phase of the wound healing process.
Product Description
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Vereshchaka Volodymyr V.
Vovk Tetiana B.
Kalashnikova Maryna V.
Ostapchenko Liudmyla I.
Raksha Nataliia H.
Stepanova Liudmyla I.
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2025-04-04
