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Information × Registration Number 0225U003633, (0118U004253) , R & D reports Title Morphogenesis of destructive-reparative processes of the brain in diseases of vascular and toxic-metabolic genesis popup.stage_title Морфогенез деструктивно-репаративних процесів головного мозку при захворюваннях судинного та токсико-метаболічного ґенезу Head Tertyshnyi Serhii Ihorovych, д.мед.н. Registration Date 24-07-2025 Organization Zaporizhzhya State Medical University popup.description1 popup.description2  The object of the study is sepsis-associated, hepatic, diabetic encephalopathy, ischemic brain infarction. The aim of the study is to optimize the pathomorphological diagnosis and clarify the links of morpho- and pathogenesis of somatogenic toxic and diabetic encephalopathies, as well as ischemic brain infarction through complex clinical and laboratory, neuromorphological, immunohistochemical and electron microscopic determination of the dynamic parameters of their morphogenesis. Research methods: Clinical and anatomical: processing and analysis of instrumental and laboratory data from the histories of diseases of deceased patients with sepsis-associated and hepatic encephalopathy; Morphological: histological, electron microscopic, histochemical, immunohistochemical methods (GFAP, GlnS, Aqp-4, MMP-9, TIMP-1, VEGF-A, VEGFR-2, CD 31, CD 105, α-SMA, Collagen IV, I and III, CD68, CD4, CD8, iNOS, Syn38, Ki67, Caspase-3), photodigital morphometry; Statistical analysis of obtained quantitative results. Scientific novelty of the obtained results: It is shown for the first time that the CLP model of severe sepsis in rats stimulates a dynamic increase in the level of AQP4, GS and GFAP in 5 regions of the brain with the greatest increase in the cortex. The AILF rat model of acute acetaminophen-induced liver failure was found to induce dynamic increases in AQP4 and GS levels in the cortex, hippocampus, caudate nucleus/shell, white matter, and thalamus with the greatest increase in the cortex. On the contrary, under the same conditions, a heterogeneous and dynamic decrease in GFAP expression was noted, with the most significant decrease in the cortex and thalamus. For the first time, AILF has been shown to potentiate dynamic, region-specific microgliosis with highest rates in the subcortical white matter, thalamus, and caudate nucleus/shell. A small percentage of cells with an amoeboid phenotype among CD68+ cells implies a partial functional failure of microglia in the setting Product Description popup.authors Avramenko Yuliia M. Voloshanska Olha O. Shuliatnikova Tetiana V. popup.nrat_date 2025-07-24 Close