Registration Number
0225U003779,
(0123U103772)
, R & D reports
Title
Biophysical characteristics of circulating metastatic cells as potential targets of antimetastatic therapy
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Дослідження впливу інгібіторів енергетичного метаболізму на виживаність та біофізичні показники клітин LLC та LLC/R9 за їх деадгезивного росту (модель циркулюючих метастатичних клітин) in vitro.
Head
Dovbeshko Halyna I., д.ф.-м.н.
Registration Date
16-08-2025
Organization
Institute of Physics of National Academy of Sciences of Ukraine
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The aim of the work is to determine biophysical and biochemical characteristics, the changes of which are correlated with the viability of circulating tumor cells and to experimentally substantiate the possibility of using these characteristics as potential therapeutic targets for antimetastatic therapy.
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Subject of study: Lewis lung carcinoma cell cultures with high (LLC) and low (LLC/R9) metastatic
potential.
The purpose of the study: Determination of biophysical characteristics and experimental substantiation of
the possibility of using these characteristics as potential therapeutic targets for antimetastatic therapy
Research methods and equipment: biochemical methods, cytofluorimetric method using labeled
monoclonal antibodies, IR and Raman spectroscopy, confocal and CARS microscopy.
Theoretical and practical results: Spectral markers of highly metastatic (LLC) and low metastatic
(LLC/R9) lung carcinoma cells under the condition of their adhesive and deadhesive growth were
determined. The total lipid fraction isolated from LLC and LLC/R9 tumor cells was analyzed using IR
spectroscopy. LLC and LLC/R9 cells were visualized under the condition of their adhesive and deadhesive
growth using confocal microscopy and CARS microscopy. Changes in the number of live LLC and LLC/R9
cells were studied during incubation of cells for 3 days of their adhesive and deadhesive growth
(experimental model of circulating metastatic cells). A comparative analysis of LLC and LLC/R9 growth
kinetics under different growth conditions was performed using mathematical modeling and the optimal
incubation period of cells for further studies was determined. The number of live cells, the level of
apoptosis, the distribution of cells by cell cycle phases (survival characteristics), and the mesenchymal
characteristics of membrane adhesion during adhesive and deadhesive growth of LLC and LLC/R9 tumor
cells were determined by flow cytofluorimetry.
Novelty: For the first time, in vitro studies of tumor cells of the same origin, but with different metastatic
potential, were carried out. Their comparative analysis made it possible to determine biophysical
characteristics that can be considered as prognostic markers of the risk of metastasis.
Product Description
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Barabash Yurii M.
Hnatiuk Olena P.
Dovbeshko Halyna I.
Karakhim Serhii O.
Kolesnyk Denys L.
Olenchuk Maryna V.
Solianyk Halyna I.
Stepanov Yurii V.
Chumak Alina V.
Shevliakova Hanna V.
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2025-08-16
