Registration Number
0225U003935,
(0123U102047)
, R & D reports
Title
Fundamentals of the influence of immunomodulators, probiotics and nanomaterials on the pathogenetic mechanisms of the development of viral and microbial diseases
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Дослідження впливу ряду сполук з антиоксидантною активністю (в т.ч. наноматеріалів) на ключові показники клітинного метаболізму: метаболічну, лізосомальну, проліферативну активність в стандартних умовах та за модельованого in vitro вірусного інфекційного процесу. Визначення імуномодулювальних, антимікробних та протизапальних властивостей пробіотичних культур та наноматеріалів, а також їх впливу на мікробіоту піхви та кишечника за розроблених тваринних моделей захворювань грибкової етіології. Скринінг та характеристика лактобактерій (ЛАБ), здатних до біосинтез монодисперсних біогенних форм наноселену Nano-Se
Head
Spivak Mykola Ya., Доктор біологічних наук
Registration Date
15-09-2025
Organization
Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine
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The purpose of the work is to determine the pathogenetic mechanisms of the development of infectious processes of viral and microbial etiology, the course of which is accompanied by the development of the body's inflammatory reaction, as well as to establish the influence of immunomodulators, probiotics and nanomaterials on the pathogenetic mechanisms of their development.
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The effect of cerium dioxide nanoparticles functionalized with amino acids on the antioxidant activity under the conditions of induction of oxidative stress by hydrogen peroxide and antiviral activity under the conditions of a simulated in vitro viral infection process was determined. The antiviral activity of the line of non-functionalized cerium dioxide nanoparticles in both studied schemes was less significant than that of nanoparticles functionalized with amino acids. The therapeutic properties of probiotic cultures were established in developed animal models of diseases of fungal etiology. Screening of LAB strains capable of bioconversion of sodium selenite with the formation of monodisperse biogenic nanoselenium (Nano-Se), which is carried out extracellularly and intracellularly, was carried out. It was established that the key factor in metabolic self-organization is the interaction between enzymes and metabolites of LAB, especially heterofermentative species that implement the pentose phosphate and phosphoketolase pathways. Aerobic biosynthesis of Se-NPs in probiotic cultures confirms the advantage of using endophytes as biocarriers. The obtained Nano-Se has effective antioxidant, immunomodulatory and antimicrobial activity, and the method of its synthesis complies with the principles of green chemistry.
Keywords: nanomaterials, lactobacteria, inflammation, immunity, selenium, nanocerium
Product Description
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Babenko Lidia P.
Bubnov Rostyslav B.
Ganova Larysa A.
Demchenko Oleksandr A.
Dubova Iryna V.
Zholobak Nadiia M.
Lazarenko Liudmyla M.
Pavlyuk Tetyana V.
Tymoshok Natalia O.
Umarova Oksana V.
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2025-09-15
