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Information × Registration Number 0225U004000, (0121U112344) , R & D reports Title Investigation of the electrical properties of nanostructured electrodes of different morphology in an electrochemical cell by impedance spectroscopy before and after biofunctionalization popup.stage_title Розробка методики виявлення специфічних послідовностей нуклеїнових кислот за допомогою спектроскопії електричного імпедансу Head Rachkov Oleksandr E., Кандидат біологічних наук Registration Date 14-10-2025 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description1 Investigate the electrical properties of nanostructured electrodes of different morphology in an electrochemical cell by impedance spectroscopy before and after biofunctionalization popup.description2 As a result of the project, ultrasensitive and highly selective hybridization DNA sensor devices were developed, capable of rapid and reliable detection of complementary nucleic acid sequences. Laboratory prototypes of these DNA sensors were created, demonstrating detection limits for target DNA ranging from 0.001 to 0.1 nM, depending on the analysis variant. The linear dynamic range of the developed sensor devices spans from 0.001 to 10 nM. Approaches to improving the selectivity of the developed hybridization DNA sensors and strategies for their repeated use have been proposed. It was demonstrated that performing the analysis at an elevated temperature—corresponding to the optimal hybridization temperature of the immobilized oligonucleotide probe with a fully complementary sequence—resulted in a lowered detection limit for complementary oligonucleotides down to 0.001 nM, as well as a significant enhancement in selectivity in the presence of potential interferents (partially complementary and non-complementary nucleic acid sequences) in the test samples. Product Description popup.authors Viktoria Peshkova Evgenia Nesterenko Tetyana Sergeyeva popup.nrat_date 2025-11-03 Close