Registration Number
0225U004000,
(0121U112344)
, R & D reports
Title
Investigation of the electrical properties of nanostructured electrodes of different morphology in an electrochemical cell by impedance spectroscopy before and after biofunctionalization
popup.stage_title
Розробка методики виявлення специфічних послідовностей нуклеїнових кислот за допомогою спектроскопії електричного імпедансу
Head
Rachkov Oleksandr E., Кандидат біологічних наук
Registration Date
14-10-2025
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
popup.description1
Investigate the electrical properties of nanostructured electrodes of different morphology in an electrochemical cell by impedance spectroscopy before and after biofunctionalization
popup.description2
As a result of the project, ultrasensitive and highly selective hybridization DNA sensor devices were developed, capable of rapid and reliable detection of complementary nucleic acid sequences. Laboratory prototypes of these DNA sensors were created, demonstrating detection limits for target DNA ranging from 0.001 to 0.1 nM, depending on the analysis variant. The linear dynamic range of the developed sensor devices spans from 0.001 to 10 nM. Approaches to improving the selectivity of the developed hybridization DNA sensors and strategies for their repeated use have been proposed. It was demonstrated that performing the analysis at an elevated temperature—corresponding to the optimal hybridization temperature of the immobilized oligonucleotide probe with a fully complementary sequence—resulted in a lowered detection limit for complementary oligonucleotides down to 0.001 nM, as well as a significant enhancement in selectivity in the presence of potential interferents (partially complementary and non-complementary nucleic acid sequences) in the test samples.
Product Description
popup.authors
Viktoria Peshkova
Evgenia Nesterenko
Tetyana Sergeyeva
popup.nrat_date
2025-11-03
