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Information × Registration Number 0225U004369, (0125U002657) , R & D reports Title Biomedical Center for Innovative Research in Neuroscience popup.stage_title Дослідження нейропротекції та синаптичної активності. Head Skybo Halyna H., Доктор медичних наук Registration Date 02-12-2025 Organization Bogomolets Institute of Physiology of the National Academy of Sciences of Ukraine popup.description1 The aim of the project is to establish а forefront research center specializing in the development of innovative approaches and methods in the field of neuroscience. The center aims to integrate modern scientific knowledge and research for a deeper understanding and treatment of brain pathologies and neurological disorders. It includes handling advanced research in the fields of neuropathology, and developing new therapeutic strategies for treating neurodegenerative diseases. popup.description2 During the intermediate stage of the project, studies were conducted to investigate the mechanisms of neuronal damage and synaptic transmission dysfunction under conditions of hypoxia, hypoinsulinemia, oxygen–glucose deprivation (OGD), as well as in models of epilepsy and Alzheimer’s disease at the molecular, cellular, and neurophysiological levels. The role of protein kinase C in hypoxia-induced depression of GABA A mediated neurotransmission was examined using paired whole-cell patch-clamp recordings with the application of chelerythrine. The effect of insulin on glutamatergic transmission in hippocampal neurons under hypoinsulinemic conditions was also examined. A 30-minute OGD protocol followed by reperfusion was optimized. Using real-time PCR, the expression of CASP-3 and TNF was analyzed 4 hours after OGD. Only initial transcriptional changes were detected at this early stage, whereas sodium propionate reduced transcript levels, indicating potential anti-inflammatory and anti-apoptotic effects. Immunohistochemical analysis revealed a reduction in the number of synaptic contacts (PSD95) after OGD. In the Alzheimer’s disease model, β-amyloid increased the levels of apoptotic and necrotic neurons to 28.5% and 20.8%, respectively. The CDK5 inhibitor roscovitine significantly reduced these values to 18.9% and 9.5%, thereby enhancing cell survival. In in vitro and in vivo epilepsy models, epileptic discharges were analyzed in the CA1 and CA3 regions of the hippocampus, and EEG monitoring was performed. Diazepam significantly reduced animal mortality, confirming the key role of the GABAergic system in survival after seizures. Product Description popup.authors Halyna H. Skybo Mariia S. Shypshyna Viacheslav B. Kulyk Olena V. Yehorova Hanna V. Dumanska Mykola S. Veselovskyi Oleh M. Tsupykov Dmytro S. Isaiev Iryna V. Lushnikova Maryna A. Patseva Nataliya M. Rozumna Vita V. Hanzha Oleksandr P. Maksymiuk Olena O. Lukianets Cherninskyi Andrii О. popup.nrat_date 2025-12-02 Close