Registration Number
0225U004773,
(0125U001298)
, R & D reports
Title
Multivariate comparative analysis of antigen-presenting niches of tumors and blood cells of patients with glioblastomas, their prognostic significance
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Оцінка мікрооточення та імунного профілю Т-клітин та молекул імунної супресії у пухлинах та клітинах периферичної крові пацієнтів з гліобластомами
Head
Onyshchenko Kateryna V., Доктор філософії
Registration Date
18-12-2025
Organization
Institute of Molecular Biology and Genetics of NAS of Ukraine
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To conduct a multivariate comparative analysis of antigen-presenting niches in tumor tissues and blood cells of patients with glioblastoma to identify key immunological markers that may be important for the prognosis of the disease.
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During the first stage of the project, a unique collection of clinical samples was established from 60 patients with suspected glioblastoma, including tumor tissue, adjacent non-tumorous brain tissue, and peripheral blood. Morphological and molecular verification of the samples was performed according to the current WHO classification, allowing the identification of IDH1 wild-type glioblastoma samples for further analysis.
Protocols for tissue and cell processing and cryopreservation were optimized, ensuring high post-thaw viability and long-term storage of samples in the project biobank. A preliminary analysis of the immune cell composition in peripheral blood and tumor tissue revealed clear differences between systemic and local immune environments in glioblastoma.
An important achievement of this stage was the establishment of glioblastoma organoid models derived from cryopreserved tumor tissue. These organoids retained key morphological and cellular markers of the original tumors, demonstrating their relevance as experimental models. Overall, the obtained results provide a solid experimental and resource platform for future studies of immune niches in glioblastoma and for the development of novel prognostic and therapeutic approaches.
Product Description
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Svitlana V. Antonenko
Oleksandr V. Zhuk
Anastasiia R. Shloma
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2025-12-18
