Registration Number
0225U005364,
(0125U001032)
, R & D reports
Title
Development of a novel amperometric biosensor for the analysis of 5-hydroxyindoleacetic acid - a biomarker of serotonin-producing neuroendocrine tumors.
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Скринінг та оптимізація культивування штамів мікроскопічних і шапинькових грибів продуцентів лаккази. Генно-інженерне конструювання штама - продуцента грибної лаккази на основі метилотрофних дріжджів Komagataella phaffii та очищення ензиму.
Head
Honchar Mykhailo V., Доктор біологічних наук
Registration Date
30-12-2025
Organization
Institute of Cell Biology of the National Academy of Sciences of Ukraine
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The aim of the research project is to develop a laccase-based amperometric biosensor for the non-invasive analysis of 5-hydroxyindoleacetic acid (5-HIAC), a biomarker of serotonin-producing neuroendocrine tumors.
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Screening of microscopic fungal strains was carried out and the most promising laccase producer was selected - Monilinia fructicola. Optimal cell cultivation conditions were studied to increase the productivity of the strain. Screening of cap fungi for the ability to synthesize extracellular laccase was carried out. Four strains - Trametes hirsuta 338, T. zonata 1525, T. maxima 1002 and Ph. hadriani 2590 are capable of secreting the enzyme above 2 U/ml. Cultivation conditions were optimized for the best producer of the fungus T. zonata 1525 and the optimal composition of the medium for maximum laccase yield (up to 18 U/ml) was determined. A purified laccase preparation was obtained from the culture medium of the fungus T. zonata 1525 and its structural and enzymatic properties were studied. A series of vectors for displaying T. versicolor on the cell surface of yeasts K. phaffii and S. cerevisiae, as well as a plasmid for expressing the intracellular form of laccase based on Ogataea polymorpha, were constructed. Immunofluorescence microscopy confirmed the localization of the fungal recombinant laccase on the cell surface of S. cerevisiae transformants.
Product Description
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Prokopiv Tetyana M.
Demkiv Olha M.
Dmytruk Olena V.
Andriy A. Sybirnyy
Dmytruk Kostiantyn V.
Oksana M. Zakalska
Andrii Y. Zakalskyi
Nataliia Y. Stasiuk
Galina Z. Gayda
Horecha Mariia V.
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2025-12-30
