Registration Number
0226U000287,
(0124U000127)
, R & D reports
Title
Increasing the efficiency of obtainment of starting materials of spring barley with pigmented grain using in vitro anther culture
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Підвищення ефективності одержання вихідного матеріалу ярого ячменю з кольоровим зерном за допомогою культури пиляків in vitro
Head
Bilynska Olena V., Кандидат біологічних наук
Registration Date
07-01-2026
Organization
Institute of Plant Breeding named after V. Ya. Yuriev of the National Academy of Agrarian Sciences of Ukraine
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The purpose is to obtain doubled spring barley haploids based on hybrids from crossing varieties and lines that are carriers of valuable grain quality traits with collection accessions with pigmented grain
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The study object was variability of collection spring barley accessions with pigmented caryopses and hybrid populations in response to androgenesis in vitro. The purpose of the study was to determine the capability of colored-grain spring barley accessions for haploid induction in anther culture in vitro, to produce androgenetic doubled haploids from hybrids derived from these accessions, to improve methods for ploidy determination in regenerated plants, and to evaluate the frequency of their spontaneous chromosome doubling. For the first time, the abilities to produce haploids and doubled haploids in anther culture in vitro were evaluated for eight spring barley accessions with pigmented caryopses from the collection of the NCPGRU. Among them, two accessions with black and yellow caryopses and medium capacities for producing androgenetic structures and regenerated plants with normal pigmentation were selected. A working collection containing spring barley accessions that combined certain valuable agronomic characteristics with capacities for production of haploids and double haploids in anther culture in vitro was enriched. Based on the results of two-year investigations aimed at estimating levels of expression of valuable agronomic traits, eight high-yielding R4 lines were selected as promising ones for registration with the NCPGRU. The possibility for growing regenerated plants in an artificial climate during the autumn-winter period using energy-saving lighting and temperature modes followed by further plant growing and obtaining seed progeny in spring-summer period in greenhouse under the natural lighting was investigated. It was found that suboptimal growth conditions fostered 90−95% survival and viability of plants. A method for ploidy determination in regenerated plants based on measurements of leaf stomatal cell length was developed to ensure highly efficient mass screening and selection of haploids for doubling their chromosome number
Product Description
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Roman L. Bohuslavskyi
Olha V. Solonechna
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2026-01-07
