Registration Number
0226U001031,
(0124U002185)
, R & D reports
Title
Development of technological principles for obtaining biodiesel from carinata oil (Brassica carinata) through lipase transesterification
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Порівняння ефективності конверсії олії B. carinata за допомогою різних комерційних ліпаз, оптимізація умов трансестерифікації з використанням ліпаз та Оцінити ефективність отримання біопалива на основі олії B. carinata в Україні.
Head
Yemets Alla І., д.б.н.
Oleksii M. Savchuk, Доктор біологічних наук
Registration Date
20-01-2026
Organization
Department for special training National academy of science of Ukraine at Kiev University
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The purpose of the proposed research work involves a bioinformatics study of the similarity of commercial and endogenous carinata lipases, the identification of the most effective lipases for the transesterification of carinata oil and the production of diesel biofuel, as well as the selection of the most promising genotypes of B. carinata according to performance indicators and features of the fatty acid composition for biofuel production. In conclusion, the project aims to estimate the volumes and prospects of obtaining biofuel based on B. carinata oil in Ukraine, based on the data obtained during the implementation of the project.
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Within the framework of the II stage of the project, a comparative analysis of the efficiency of lipase-catalyzed transesterification of Brassica carinata oil was carried out using a number of commercial lipases of different origin. Lipases obtained from representatives of the genera Candida, Thermomyces, Rhizomucor and other microorganisms, which are widely used in industrial biotechnological processes, were studied. Significant differences in catalytic activity, substrate specificity and optimal reaction conditions for different lipases were established.
Based on experimental data, lipases with the highest efficiency of converting triglycerides of B. carinata oil into ethyl esters of fatty acids were identified. Key transesterification parameters, in particular reaction duration, temperature and catalysis conditions, were optimized, which ensured a high and stable yield of biodiesel. The obtained results confirm the feasibility of using the enzymatic approach as an environmentally safe alternative to chemical methods.
Based on data on the productivity of the studied B. carinata genotypes and the efficiency of lipase-mediated transesterification, the potential for biofuel production in Ukraine was assessed. It was shown that B. carinata is a promising crop for the formation of a renewable raw material base for bioenergy and increasing the energy independence of the state.
Product Description
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Rostyslav Y. Blume
Anastasiia M. Rabokon
Olesia O. Kalmykova
Daryna I. Krenytska
Vitalii Y. Hotsuliak
Kalashnikova Maryna V.
Nataliia S. Nikitina
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2026-01-20
