Registration Number
0226U001077,
(0125U002005)
, R & D reports
Title
Study of the interaction of polymer composites and nanocarriers with microbial enzymes for biosensor analysis of the quality of alcohol-containing products
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Структурно-морфологічна характеристика та дифузійні властивості уреасильних з халькогенідними кластерами та фотозшитих з різним ступенем зшивки полімерів і наноносіїв (електропровідні металеві наночастинки) та їх здатність до іммобілізації нанозимів та біосумісності із ферментами.
Head
Klepach Halyna M., Кандидат біологічних наук
Registration Date
21-01-2026
Organization
Drohobych Ivan Franko State Pedagogical University
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The goal of the project is to study the mechanisms of interaction of a polymer matrix with electrically conductive nanoparticles, nanozymes (nanoparticles – mimetics of natural enzymes) and immobilized recombinant enzymes to develop new and improve existing amperometric biosensors for the analysis of ethanol, methanol and other alcohols in alcohol-containing products.
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The structural and morphological characteristics and diffusion properties of selected ureasil with chalcogenide clusters and photocross-linked with different degrees of crosslinking polymers and nanocarriers (conductive metal nanoparticles and nanozymes) were studied and the conditions under which they are able to retain nanozymes and are biocompatible with enzymes were described. Metal nanoparticles (NPs) nCuAu and nCu (nanozymes – NZ) were synthesized, their morphology was studied by scanning electron microscopy. Electrodes modified with NZ were designed and characterized. Methods for immobilizing alcohol dehydrogenase (ADH) on the surface of a NZ-modified graphite electrode (GE) using photopolymers were optimized. Analytical characteristics of the created bionanosensor (ADH/NZ/GE) were studied. The designed bionanosensor was tested for determining the ethanol content in real samples, in particular, in the Isabella wine. The presented sensory data were compared with the results obtained using the reference enzymatic kit “Alcotest”. A close correlation was found with the concentration of ethanol in real samples of alcoholic beverages, estimated using the biosensor and reference methods.
Product Description
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Svitlana Y. Voloshanska
Hoivanovych Nataliia K.
Taras S. Kavetskyi
Nataliia Y. Stasiuk
Mykhailo V. Honchar
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2026-01-21
