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Information × Registration Number 0226U001691, (0124U003739) , R & D reports Title To investigate the cytomorphological, immunophenotypic, cytogenetic and molecular-cytogenetic features of leukemic cells and factors of the tumor microenvironment in BCR/ABL-positive acute leukemias and to evaluate the relationship with clinical manifestations, course and response to treatment popup.stage_title Встановити клінічне значення комплексу цитоморфологічних, імунофенотипових, цитогенетичних та молекулярно-цитогенетичних ознак при BCR/ABL-позитивних гострих лейкеміях Head Shalay Olha O., Кандидат медичних наук Registration Date 29-01-2026 Organization State institution "Institute of blood pathology and transfusion medicine of Nataional Academy of Medical scinces of Ukraine" popup.description1 To determine the characteristic features of the morphological structure, antigenic, cytogenetic and molecular-cytogenetic profile, functional characteristics of blast cells, their microenvironment and to clarify their relationship with the clinical and hematological course and response to treatment in acute BCR/ABL-positive leukemias popup.description2 The object of the study was 55 patients with acute leukemia (AL). The aim of the work was to determine the characteristic features of the morphological structure, antigenic, cytogenetic and molecular-cytogenetic profile, functional characteristics of blast cells, their microenvironment and to clarify their relationship with the clinical and hematological course and response to treatment in acute BCR/ABL-positive leukemias. Clinical, cytological, cytochemical, immunophenotypic, cytogenetic, FISH, biological, statistical research methods were used. The analysis of clinical and hematological features, morphological, immunophenotypic, cytogenetic and functional features of blast cells was conducted. The concentration of cytokines was investigated. Cytogenetic rearrangements of various kinds associated with B-cell ALL were detected in 73% of patients, namely: BCR/ABL1 gene, t(9;22)(q34;q11), trisomies 4, 6, 8, 14, 17, 21, 11q23/KMT2A rearrangements, high hyperdiploidy, others. The most common abnormalities were t(9;22)(q34;q11) and/or BCR/ABL1 gene, that were observed in 27% of patients. Additional aberrations, besides t(9;22)(q34;q11), were observed in 60% of patients, namely: - 7, +8, +17, del(7)(q31), del(11)(q23), t(1;11)(q25;q23), +der(22)t(9;22)(q34;q11). The variant of AL was confirmed according to the WHO classification (2022). Patients were classified into risk groups based on cytogenetic analysis. The levels of TGFβ1 were determined, and it was established that HUVEC endothelial cells are capable of selectively forming adhesive intercellular contacts depending on the composition of the inducing medium and the type of leukemic cells (K562 or L1210). The results of the work were implemented in the SI "Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine" and MNE "Lviv Territorial Medical Union "Clinical Hospital for Planned Treatment, Rehabilitation and Palliative Care". Scope is oncohematology. Product Description popup.authors Valeriya V. Krasivska Olha O. Shalai Rymar Mariia M. Kostyk Khrystyna Yaroslavivna Yasnyskyi Zenovii I. Volodymyr L. Matlan Yuriy S. Karol Zotova Olena V. Barilka Vira A. Shmyhelska Solomiia M. popup.nrat_date 2026-01-29 Close