Registration Number
0226U001702,
(0123U100317)
, R & D reports
Title
Improvement of diagnosis and treatment of protozoan intestinal diseases in military personnel
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Удосконалення діагностики і лікування протозойних кишкових хвороб у військовослужбовців
Head
Pokhyl Serhii I., Доктор медичних наук
Registration Date
29-01-2026
Organization
State Institution "I. Mechnikov Institute of Microbiology and Immunology National Academy of Medical Sciences of Ukraine"
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Increasing of the efficiency for protozoan intestinal diseases diagnosis and therapy in military personnel
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Microscopic analysis of fecal samples collected from 705 service members in Northern Ukraine demonstrated an 11.5% prevalence of intestinal parasitic infections. All detected infections were attributed to protozoan parasites, comprising 85.4% pathogenic and 14.6% commensal species. Blastocystis sp. and Dientamoeba fragilis were recognized as dominant pathogens 94.2%, Giardia lamblia 5.8% of invasions in MP. It has been established that routine clinical assessment cannot distinguish the clinical presentation of intestinal protozoan infections from that of gastrointestinal diseases of other etiologies. Consequently, diagnostic confirmation must rely on specific laboratory investigations targeting pathogen detection. To optimize microscopic diagnosis of PID in MP a new method staining wet smears of FS has been developed using an original combined liquid dye - mixture of equal volumes of Betaiodine, 1% aqueous solution fast green and glycerin. It was found that the diagnostic efficiency of enzyme-linked immunosorbent assay is limited, and the microscopic method (MM) and real time multiplex polymerase chain reaction (MP-PCR-RT) have high accuracy (92.9% and 99.4%, respectively) and specificity (97.3% and 100%, respectively), however, the sensitivity of MM (66.7%) is significantly lower than that determined for MP-PCR-RT (96.0%). An economically viable and practically accessible microtube technology for growing Blastocystis sp. and D. fragilis in RPMI 1640 has been developed, which has made it possible for the first time to study the susceptibility of regional strains of these parasites to the action of metronidazole (MTZ), co-trimoxazole (COT), nitazoxanide, paramomycin, ivermectin, and loperamide. To increase the efficacy of etiotropic therapy in service members with blastocystosis, dientamoebiasis (or coinfections), it is suggested to utilize MTZ (first-line) and COT (alternative second-line), following standard dosage guidelines.
Product Description
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Pokhil Sergiy
Kirichenko Igor Ivanovich
Chyhirynska Nila A
Kostyria Iryna A.
Tymchenko Helena
Mykhailo S. Biriukov
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2026-01-29
