Registration Number
0226U001874,
(0125U001672)
, R & D reports
Title
Improvement of medical care for wounded people who require complex surgical intervention to restore lost bone fragments of critical size" (IPM-2025/4). Section 2
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Виготовлення біополімерних матеріалів мікробного походження для створення композицій з біоактивною керамікою
Head
Liliia V. Avdieieva, Доктор медичних наук
Registration Date
02-02-2026
Organization
Institute of Microbiology and Virology named after D. K. Zabolotny of the National Academy of Sciences of Ukraine
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To obtaine an experimental series of polyhydroxyalkanoates produced by strains of bacteria of the Bacillaceae family for the
creation of composites with calcium phosphate biomaterials.
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Optimized cultivation conditions for the strain Priestia endophytica CHAES 5_14 were established for the production of polyhydroxyalkanoates (PHAs). The synthesized PHA was identified as poly-β-hydroxybutyrate (PHB) with an average molecular weight of 181 kDa and thermal characteristics including a glass transition temperature of 2.3 °C, a crystallization temperature of 74.6 °C, melting temperatures of 155 and 164.5 °C, and a degradation temperature of 271 °C. A composite with hydroxyapatite (HA) was obtained using the solvent-casting method. Its mechanical properties were almost twofold lower than those of neat PHB: tensile strength of 14.3 ± 2 MPa with an elongation at break of 8.5 ± 1.2%, compared to 21.2 ± 3 MPa and 12.5 ± 4%, respectively. The biocompatibility of PHB and PHB/HA was demonstrated in vitro using the MA-104 cell line and in vivo in Wistar rats. Approaches for the fabrication of biocompatible composites with calcium phosphate materials (CPMs) for medical applications were developed. It was shown that pressing CPMs under a press onto a PHB film pre-wetted with chloroform yielded the best results for the formation of PHB/CPM composites. However, the non-uniform distribution of CPM particles over the PHB film surface indicates the need for further development of CPM deposition methods.
Product Description
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Kharkhota Maksim A.
Maksym S. Kharchuk
Kisten Oleksandr H.
Natalia I. Koretska
Alina V. Kharchuk
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2026-02-02
