Registration Number
0226U002059,
(0121U108357)
, R & D reports
Title
Development of a methodology for determining the resistance of enterobacteria to antibiotics of the group of beta-lactams and fluoroquinolones in biofilms using molecular genetic methods
popup.stage_title
Розроблення методології визначення резистентності ентеробактерій до антибіотиків групи бета-лактамів та фторхінолонів у складі біоплівок з використанням молекулярно-генетичних методів
Head
Polina S. Yurko, Кандидат ветеринарних наук
Registration Date
05-02-2026
Organization
National Scientific Center Institute of Experimental and Clinical Medicine
popup.description1
The aim of the project is to determine the ability of field isolates of enterobacteria to form biofilms and to study their resistance to antibacterial drugs of the group of beta-lactams and fluoroquinolones in this form of existence.
popup.description2
The study investigated the ability of field isolates of Enterobacteriaceae (Salmonella spp., Escherichia coli, Proteus spp., Citrobacter spp., Klebsiella spp.) to form biofilms and analyzed their resistance to beta-lactam antibiotics and fluoroquinolones in both planktonic and biofilm-associated states. A high prevalence of biofilm formation was observed, along with the detection of multiple antimicrobial resistance genes, including determinants associated with beta-lactam and fluoroquinolone resistance.
A comparative analysis of phenotypic and genotypic resistance profiles demonstrated the critical role of biofilms in the persistence and dissemination of resistance genes. Horizontal transfer of resistance determinants between different genera of Enterobacteriaceae was experimentally confirmed to occur exclusively under biofilm conditions. Within the framework of this study, molecular genetic methods were developed, optimized, and validated, making them suitable for application in veterinary diagnostic laboratories and antimicrobial resistance monitoring programs.
Product Description
popup.authors
Arefiev Vasyl L.
Biloivan Oleksandr V.
Didyk Tetiana B.
Oksana Zlenko
Maryna Kit
Anton Gerilovych
Yana Berezhna
popup.nrat_date
2026-02-05
