Registration Number
0226U002106,
(0121U108358)
, R & D reports
Title
Development of a methodology for the recombinant control samples of nucleic acids and proteins of infectious agents construction for use in veterinary laboratory diagnostics
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Створення методології розробки рекомбінантних контрольних зразків нуклеїнових кислот і протеїнів збудників інфекційних хвороб для використання у лабораторній ветеринарній діагностиці
Head
Polina S. Yurko, Кандидат ветеринарних наук
Registration Date
07-02-2026
Organization
National Scientific Center Institute of Experimental and Clinical Medicine
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Development of methodologies for the construction of recombinants, in particular, methodologies for obtaining plasmid recombinant positive controls for the detection of bacterial pathogens (mycoplasmosis) and viral infections (ASF viruses, bovine nodular dermatitis and Newcastle disease) using various PCR formats; and methodology for obtaining recombinant antigen-expressing vectors producing ASFV proteins.
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A set of studies aimed at developing safe and affordable recombinant materials for laboratory diagnosis of animal infectious diseases was carried out. Recombinant plasmid DNAs were obtained and validated as positive controls for polymerase chain reaction assays targeting African swine fever virus and lumpy skin disease virus. These controls demonstrated high analytical sensitivity and stability, while eliminating the need to handle live or inactivated pathogens.
A separate part of the work focused on the production and evaluation of recombinant proteins of African swine fever virus for serological applications. Among the tested proteins, p32 showed the most pronounced antigenic properties and proved to be suitable for use in enzyme-linked immunosorbent assay. The developed ELISA protocol showed high specificity, reproducibility and reliable discrimination between positive and negative sera.
In addition, a functionally active recombinant Pfu DNA polymerase was produced and tested in PCR assays. The obtained results were summarized in methodological and technological guidelines, which can be implemented in diagnostic laboratories and biotechnology facilities, contributing to improved biosafety and sustainability of veterinary diagnostics in Ukraine.
Product Description
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Anton P. Gerilovych
Biloivan Oleksandr V.
Didyk Tetiana B.
Oksana B. Zlenko
Vasyl L. Aref'iev
Maryna Y. Kit
Yana M. Berezhna
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2026-02-07
