Registration Number
0226U002147,
(0125U000650)
, R & D reports
Title
Physiological-genetic and epigenetic components of heterologous gene expression in biotechnological plant systems
popup.stage_title
Створенння генетичних векторів та генетична трансформація рослин, клонування послідовностей цільових генів, а також регуляторних послідовностей, які здатні поліпшувати рівень експресії перенесених генів
Head
Kuchuk Mykola V., д.б.н.
Registration Date
09-02-2026
Organization
Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine
popup.description1
The aim of the study is to investigate the influence of various epigenome modifiers and regulatory elements in genetic constructs on the efficiency of plant genetic transformation and expression of transferred genes, as well as the effect of genetic transformation and epigenome modifiers on the synthesis of secondary metabolites in plants.
popup.description2
Object – plant in vitro systems capable of synthesizing recombinant proteins and biologically active compounds.
Aim – to investigate molecular, physiological-biochemical, and morphogenetic processes that determine the efficiency of expression of transferred genes in plant systems. This includes cloning and functional evaluation of structural and regulatory sequences, development and analysis of genetic vectors, as well as studying the stability of transgene expression, morphogenesis, and the regeneration potential of various plant objects. Particular attention is paid to the use of transformed plants as sources of biologically active compounds and as platforms for the “green” synthesis of metal nanoparticles.
Research methods: in vitro culture, Agrobacterium-mediated transformation, vector construction, Golden Gate molecular cloning, PCR/RT-PCR, FTIR spectroscopy, transmission electron microscopy, surface plasmon resonance, and analysis of data from the online database QuinoaDB.
During the study, a TVCV-based vector for expression of the infα2b gene was created, providing a higher level of interferon accumulation compared with the PVX system. Fusion with calreticulin and a His₆ tag increased the efficiency of protein secretion and purification. A vector system with two reporter genes for soybean transformation using A. tumefaciens GV3101 was developed, and regeneration was optimized. Stable expression of colicin in transgenic kale lines with a heterosis effect in the T1 generation was demonstrated. A high potential of the Lemnaceae family for recombinant protein expression was established. Genes of Amaranthus hypochondriacus associated with organogenesis were identified, and a positive effect of polyethylene glycol on tomato regeneration was confirmed. The prospects of “green” synthesis of silver and gold nanoparticles from extracts of transformed roots of Artemisia annua were demonstrated.
Product Description
popup.authors
Nifantova Svitlana M.
Голубенко Анастасія Володимирівна
Symonenko Yurii V.
Peterson Anton A.
Maistrov Petro D.
Olha V. Bulko
Makhorina Olha K.
Parii Myroslav Fedorovych
Vasylenko Maksym Yu.
Лучаківська Юлія Сергіївна
Ya. R. Sindarovska
Дуплій Володимир Павлович
Yaroshko Olha M.
Matvieieva Nadiia Anatoliivna
Ratushniak Yakiv I.
Taisa A. Bohdanovych
Anatolii M. Shakhovskyi
Anastasia V. Kirienko
Lyubov O. Zharkova
popup.nrat_date
2026-02-09
