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Information × Registration Number 0226U002189, (0121U100495) , R & D reports Title Investigation the features of the sheep microsatellites polymorphism domestic selection popup.stage_title Дослідження особливостей поліморфізму мікросателітів овець вітчизняної селекції. Head Iovenko Vasyl M., Доктор сільськогосподарських наук Registration Date 10-02-2026 Organization MF Ivanov Askania-Nova Institute of Steppe Animal Husbandry - National Scientific Breeding and Genetics Center for Sheep Breeding popup.description1 Investigation of microsatellite loci polymorphism in the Ascanian selection sheep and determination of their use possibility to determine: the genetic structure of existing and newly sheep created breeds and types; the phylogenetic level of domestic and foreign selection individual gene pools; monitoring of microevolutionary processes in sheep populations in the Ukraine south; examination of the breeding animals’ origin. Obtaining new knowledge on the molecular mechanisms of the sheep breeds' biodiversity formation and types under the influence of the selection and breeding work. popup.description2 The features of STR locus polymorphism were investigated in Askanian Karakul (AKP), Askanian Fine-Fleeced (AFP), and Askanian Meat-and-Wool (AMWP) sheep breeds. In total, 34 alleles were identified across five microsatellite loci (OarFCB20, INRA172, INRA063, MAF214, CSRD247) in the studied populations of Askanian-bred sheep. The number of alleles per locus ranged from 5 (OarFCB20) to 9 (MAF214), indicating a high level of polymorphism of these markers. All analyzed microsatellite loci were polymorphic. The loci INRA172, INRA063, and MAF214 proved to be the most informative for breed differentiation and can be recommended for further genetic certification and animal identification. For a more in-depth assessment of the genetic structure of populations, it is advisable to expand the marker panel by including additional STR loci and to increase the number of animals studied from different production types. Analysis of the experimental data indicates the distinctiveness of the genetic profiles of the studied populations based on microsatellite data, with significant differences observed among them. The Askanian Karakul and Askanian Fine-Fleeced breeds were the most genetically related, whereas the Askanian Meat-and-Wool breed formed a separate, independent genetic subcluster; however, within the Askanian-bred sheep, it retains common elements of the shared gene pool. Recommendations for microsatellite typing and determination of allele pools of domestically bred sheep populations have been developed. Product Description popup.authors Guzevayiy Oleg Evgenovich Dzitsiuk Valentyna V. Kostiantyn V. Skrepets Yakovchuk Hanna O. Olena Y. Kraieva popup.nrat_date 2026-02-10 Close