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Information × Registration Number 0306U003144, 0105U002652 , R & D reports Title The investigation of the molecular-genetical nature of mutations which are caused to the men's and women's infertility popup.stage_title Дослідження спектру та розповсюдженості мутацій у генах трансмембранного регуляторного білка муковісцидозу і частоти мікроделецій довгого плеча Y-хромосоми у чоловіків з важкими формами безпліддя Head Barilyak I.R., Registration Date 08-02-2006 Organization Research Centre for Radiation Medicine popup.description2 Object: samples of peripheral blood of the men with infringements spermatogenesis. The purpose: research of mutations in genes transmembrane regulatory protein of cystofibrosis (CFTR) and microdeletions of a long shoulder Y-chromosome at the patients with infringements spermatogenesis and finding - out of a role these mutations in the hereditary forms man's infertility. Methods: the genealogic analysis and construction of family trees, allocation and cleaning of an desoxyrybonucleic acid (DNA), polymerase chain reaction (PCR), the restriction analysis, electroforetic division of DNA fragments, methods of biocomputer science and variational statistics of qualitative attributes. In work the data on a molecular-genetic nature of damages of genes resulting to infringements gametogenesis at the men are submitted. The research of a nature and spectrum deletions in family of genes AZF in group of the men with nonobstructive by the forms infertility is carried out. The association of a genotype with stages ofinfringement gametogenesis is investigated. For the first time in a population of Ukraine for study of a role certain mutation of a CFTR gene in pathogenesis man's infertility the study of prevalence most widespread mutations of a CFTR gene in group of the patients is carried out. Product Description popup.authors popup.nrat_date 2020-04-02 Close