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Information × Registration Number 0320U002008, 0115U004346 , R & D reports Title Biosafety and efficiency of interaction of inert metals nanoparticles with bone marrow of mesenchymal stem cells popup.stage_title Визначення біобезпеки та цитотоксичного впливу наночастинок золота на моношаровій та сфероїдній культурі пухлинних клітин. Head Sydorenko Mychail V., Registration Date 09-07-2020 Organization "Department of Biotechnical Problems in Diagnostic of Institute of Problems of Cryobiology and Cryomedicine National Academy of Sciences of Ukraine" popup.description2 Abstract. 30 р., fig. 7, tab. 1, sources 19. Object of study - bone marrow mesenchymal stem cells (MSCs) and platinum nanoparticles. The aim of this phase of the project was to study the effect of platinum nanoparticles on the morphological and functional characteristics of cryopreserved bone marrow mesenchymal stem cells. The objectives of this stage of the work were to study the effect of platinum nanoparticles on the ability to proliferate, colony formation, synthesis of type I collagen, the state of apoptosis / necrosis and the receptor profile in cells. Research methods - morphological, cytofluorimetric, immunohistochemical, spectroscopic. Results of research. The results obtained in the work indicate that during the cultivation of MSC KM in the presence of PtNPs, vimentin synthesis in the cytoplasm of MSC decreases from 98.0%, in the control to 74.0%, at 10.0 ?g / ml. Upon incubation of MSC CM with PtNPs, an increase in the concentration of platinum correlates with an increase in the expression of high molecular weight cytokeratins to 99.4%. When culturing MSC KM in the presence of PtNPs, there is no increase in the accumulation of pro-apoptotic p53 protein. When cultured with PtNPs in concentrations from 2.0 to 10.0 ?g / ml, an increase in cells expressing EpCAM ++ / + cells was observed, from 73.7% to 88.6%, respectively. Based on the results of our studies, we conclude that PtNPs have, first, a cytotoxic effect on MSCs, at concentrations of 6.0 and 10.0 ?g / ml. And secondly, they stimulate increased expression of mesenchymal cells of markers of epithelial genesis, namely cytokeratins and EpCAM, and reduce vimentin synthesis. Based on the absence of p53 accumulation in the cells, we assume that the concentrations of PtNPs are not affected by the incubation time used. Key words: mesenchymal stem cells, platinum nanoparticles, cytokeratin receptors, p53 protein, apoptosis, EpCAM, vimentin. Product Description popup.authors Безуглий С.В. Гергелюк Т.С. Перепелиціна О.М. Сидоренко Михайло Васильвич Угнівенко А.П. Ястребова О.В. popup.nrat_date 2020-10-01 Close