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0410U004537,
к.б.н.
20-10-2010
o
Biofilm of heterotrophic bacteria as a factor of protection coating Polyken 980-25 biodeterioration
Iumyna Iuliia Mychailivna,
popup.head Kozlova Iryna Panasivna
popup.opponent Курдиш Іван Кирилович
popup.opponent Радченко Ольга Сергіївна
1. Дисертація на здобуття наукового ступеня кандидата біологічних наук за спеціальністю 03.00.07 - мікробіологія. - Інститут мікробіології і вірусології ім. Д.К. Заболотного НАН України, Київ, 2010. Дисертація присвячена вивченню формування і функціонування біоплівок на поверхні клейової основи захисного поліетиленового покриття "Полікен 980-25". Досліджено якісний склад природного угрупування гетеротрофних бактерій, яке зруйнувала бутилкаучуковий шар покриття Полікен 980-25. Воно складається із Pseudomonas pseudoalcaligenes 109, Arthrobacter flavescens 102 і Bacillus subtilis 140. За допомогою КЛСМ досліджено архітектоніку біоплівок моно- і бінарної культур Pseudomonas pseudоalсaligenes 109, Arthrobacter flavescens 102. Дослідження динаміки утворення біоплівок моно- і бінарною культурами показало наявність певних стадій формування біоплівки. Визначено питоме продукування синтезу ЕПК, загальну кількість білку та вуглеводів у ЕПК за різних моделей росту. Вивчено моносахаридний склад ЕПК. Моносахаридний склад ЕПК біоплівки більш різноманітний ніж у планктоні. Ксилоза була виявлена тільки у ЕПК біоплівок моно- та бінарних культур. Показано, що міцність до розриву захисного покриття Полікен 980-25 під впливом біоплівок, сформованих моно- та бінарними культурами значно знижуються. Механізм біодеградації бутилкаучукового шару під впливом моно- і бінарної біоплівок відбувається внаслідок окисної деструкції. Запропоновано можливий механізм біодеградації бутилкаучукового шару покриття Полікен 980-25.
2010-10-20
2020-04-04
Updated: 2025-12-08
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