Registration Number
0212U001740,
0110U006123
, R & D reports
Title
Fundamental foundations multigene therapy for mass pathology with complications
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Head
Kordium Vitalii Arnoldovich, Доктор біологічних наук
Registration Date
13-03-2012
Organization
Institute of Molecular Biology and Genetics
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The goal of this project is investigation of the processes which will be take place during the two human gene delivery - insulin and main protein component of high-density lipoproteins apolipoprotein A1(Аpо АI) - on model systems in vitro and in experimental animal organisms. We expect that reproduction of insulin synthesis in organism (wherewith creation of insulin producer cells in the liver) and high-density lipoproteins higher level synthesis, that is one of the key constituent for vessels maintenance, will enable to create scientific and technological prerequisites for medical treatment of Type 1 diabetes and recovery of the organism from vascular lesions, which are complications of main pathology. For the period under review in accordance with plan the integrative plasmid vectors with hight level human ApoA1 expression it have been constructed. The transfection experiment with use nonviral gene delivery system on basis of lactosilated polyethylenimine have been performed, the transgenic various mammalian origin cell lines, which stably express the target human proteins were obtained. The features of ectopic expression of ful-size transgenes human preproinsulin and apolipoprotein A1 in mammalian cells in vitro have been research.
Product Description
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Іродов Дмитро Михайлович
Гулько Тамара Павлывна
Ковальчук Марія Вікторівна
Моргунов Павло Вікторович
Похоленко Яніна Олександрівна
Римар Світлана Юхимівна
Топорова Олена Карнеліївна
Шпильова Світлана Павлівна
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2020-04-02
