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Information × Registration Number 0213U004967, 0110U006128 , R & D reports Title Development of fundamentals methods rof cell therapy of heart pathologies popup.stage_title Head Lyubov L. Lukash, Доктор біологічних наук Registration Date 16-01-2014 Organization Institute of Molecular Biology and Genetics popup.description2 Were examined gene expression involved in the canonical Wnt signaling (TSF-4, Axin2, b-katenin) and genes that are controlled by this signaling pathway (sFos, Cyclin D1, cMyc, Sh43) in the myocardium of animals aged 1, 3 and 6 months, provided that embryonic deletion of one allele kardiospetsyfichnoyi b-catenin gene. To investigate the level of gene expression using RT-PCR method in real time, normalizing the data obtained relative expression level of a gene GAPDH. It was found lower levels of gene expression TSF-4 and higher levels of gene expression b-catenin and Axin2 in the myocardium of mutant animals of all age groups, indicating that the inhibition of the canonical Wnt-signaling. In the analysis of genes that are under the control of the canonical Wnt-way has been found in violation of their expression in animals with lack of one allele of ?-catenin gene. It should be noted that these animals only sFos level of gene expression, which is known from the literature data is controlled only investigated signal-regulatory way in all age groups was lower compared with that in control animals. We discuss the importance of the proper functioning of the canonical Wnt-signaling for growth and development of the adult myocardium of the heart. Product Description popup.authors Бобик Василь Васильович Костецький Ігор Євгенович Мацевич Лариса Леонідівна Півень О.О. Сидорик Людмила Леонідівна popup.nrat_date 2020-04-02 Close