Registration Number
0222U000500,
0118U001714
, R & D reports
Title
To determine the peculiarities of chemical and radiation factors genotoxic influence on radiotherapy cytogenetic effects in cancer patients in vivo and ex vivo
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Head
Maznyk Nataliia O., д.б.н.
Registration Date
11-01-2022
Organization
State Organization "Grigoriev Institute for Medical Radiologiy of the National Academy of Medical Sciences of Ukraine"
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Object of study: cytogenetic effects in cultured peripheral blood lymphocytes of cancer patients with various tumor localizations during therapeutic radiation exposure followed by chemotherapy. The aim: to assess the effect of previous chemoradiotherapy on the character of radiation- and chemical-induced cytogenetic effects during radiation therapy in vivo and ex vivo depending on the mutagenic factor and tumor localizations. Research methods and techniques: cytogenetic analysis of human blood cultured lymphocytes, statistical methods of data processing, microscopes Axioskop, Micros MC300X, Olympus CX41 and Olympus, system ASIS. Theoretical and practical results: the influence of previous treatment on the accumulation of radiation-induced aberrations in cancer patients depending on the tumor localizations was found. The increase of radiation-induced aberrations from beginning to end of treatment in patients with lung cancer with previous chemo- or radiation therapy, and less pronounced changes in these parameters in patients with head and neck tumors were demonstrated. Against the background of the absence of a pronounced effect of chemotherapeutic treatment on the frequency of radiation-nonspecific chromatid type damages a dependence of these parameters on the tumor localizations was observed. In ex vivo model experiments the regularities of the yield and distribution of chromosome aberrations in irradiated patients’ lymphocytes against the background of chemotherapeutic drugs effect were found. Novelty: the quantitative yield and quality range of cytogenetic damage in blood lymphocytes of patients with lung cancer and head and neck cancer before and during radiation therapy in the presence of breaks in exposure or in the presence of previous chemoradiation therapy were identified. The effectiveness of implementation: methodological approach of the study was implemented in institute’s clinic. Area of application: radiobiology, radiation therapy.
Product Description
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Artamonova Neonila Olehivna
Artiukh Serhii Volodymyrovych
Bilozor Nataliia Volodymyrivna
Haiseniuk Larysa Oleksandrivna
Hukova Inna Mykolaivna
Dashkova Svitlana Anatoliivna
Zabobonina Larysa Volodymyrivna
Kolpakova Liudmyla Kuzmivna
Krugova Iryna Mykolaivna
Mikhanovskyi Oleksandr Albertovych
Niemykina Nadiia Viktorivna
Pshyshevska Liudmyla Anatoliivna
Sviatenko Nina Ivanivna
Sypko Tetiana Serhiivna
Slobodianiuk Olha Volodymyrivna
Starenkyi Viktor Petrovych
Sukhin Vladyslav Serhiiovych
Kharchenko Yuliia Volodymyrivna
Cherkasko Liudmyla Volodymyrivna
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2022-03-09
