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Information × Registration Number 0222U001916, 0121U112086 , R & D reports Title Investigation of photophysical and photochemical properties and interaction with RNA-containing virus membranes in order to inactivate them. popup.stage_title Head Yashchuk Valerij M., Доктор фізико-математичних наук Registration Date 04-02-2022 Organization Taras Shevchenko National University of Kyiv popup.description2 Object of research: Spectral-luminescent properties of viruses and their components (RNA and proteins); electronic processes in viruses and their components, including energy transfer. Objective: Investigation of electronic processes in viruses and their RNA and proteins. Research methodology: measurement of absorption, fluorescence and phosphorescence spectra of viruses and their components (RNA and proteins). The process of virus accumulation on sensitive cell cultures was optimized, pure suspensions of RNA-containing viruses were obtained, and viral RNA and viral protein preparations were purified. The absorption, fluorescence, and excitation spectra of fluorescence, phosphorescence, and phosphorescence excitation of the RNA-containing infectious pancreatic necrosis virus (IPNV) virions and the isolated double-stranded viral RNA and capsid proteins up to 220 capsules up to 800 proteins were recorded. Viral RNA and capsid proteins have been found to contribute to virion uptake. However, RNA does not manifest itself in the spectra of their fluorescence and phosphorescence. It was found that the fluorescence and phosphorescence of virions are caused mainly by proteins of the capsid shell of the virus. The attenuation kinetics of different phosphorescence bands of viral RNA were investigated in order to identify them. In the spectrum phosphorescence of viral RNA revealed at least two systems of bands that have excellent attenuation kinetics.  Product Description popup.authors Artemenko Oleksandr Yu. Berezovska Nataliya I. Buchatsʹkyy Leonid Р. Zaloilo Olga V Kravchenko Vladyslav M. Losytsʹkyy Mykhaylo Yu. Nikolayeva Olena A. Navozenko Oleksandr M. Rud' Yuriy P. Shafarenko Kateryna G. popup.nrat_date 2022-03-09 Close