Registration Number
0223U001294,
0122U001909
, R & D reports
Title
Pathology of the joints, caused by metabolic disorders in the post-hepatic period
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Head
Savchuk Oleksii M., Доктор біологічних наук
Registration Date
27-01-2023
Organization
Taras Shevchenko National University of Kyiv
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The object of the study: biochemical indicators of blood and synovial fluid associated with the development of osteoarthritis (OA) in patients who were sick with COVID-19.
The purpose of the work: to analyze the key biochemical indicators that characterize the metabolic state of the body of patients with osteoarthritis of the knee joints, after the SARS-CoV-2 infection, and to establish the mechanisms of the development of post-covid disorders.
Research methods: biochemical: spectrophotometric and fluorimetric, molecular-biological - polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), methods of statistical data processing.
A comprehensive study of biochemical indicators of the degree of local and systemic inflammation, the intensity of free radical oxidation processes, the functioning of the antioxidant defense system, the proteolysis system, the level of expression of genes involved in the development of joint pathology, and the content of cartilage degradation markers in the blood and synovial fluid of OA patients was conducted. have undergone SARS-CoV-2 infection.
An increase in the concentration of C-reactive protein and the content of molecules of medium molecular weight, which are an indicator of the development of endogenous intoxication and the manifestation of an inflammatory reaction, accompanied by the intensification of free radical processes: an increase in the concentration of reactive oxygen species (ROS; superoxide radical and hydrogen peroxide), products of lipid peroxidation and proteins (diene conjugates, TBC-active compounds and Schiff bases), and a decrease in the activity of antiradical enzymes (superoxide dismutase and catalase) in the blood plasma and synovial fluid of patients with OA after SARS-CoV-2 infection, compared to patients with joint pathology , who did not suffer from COVID-19.
Product Description
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Vereshchaka Volodymyr V.
Halenova Tetiana I
Dvorschenko Kateryna O.
Koval Tetyana V.
Krenytska Daryna I.
Rachkovska Antonina M.
Falalieieva Tetiana M.
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2023-01-27
