Registration Number
0223U001295,
0119U102341
, R & D reports
Title
To study the mechanisms of optimization of bone repair depending on the recipient’s age under the conditions of allogeneic bone grafts application in combination with mesenchymal stem cells and biologically active blood plasma factors.
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Head
Korzh Mykola O., Доктор медичних наук
Registration Date
27-01-2023
Organization
State institution "Sytenko Institute of Spine and Joint Pathology National Academy of Medical Sciences of Ukraine"
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Object: bone regeneration in rats of different ages when the defects are filled with bone allografts in combination with mesenchymal stem cells (MSCs) or growth factors of blood plasma (GFBP). Purpose: to study the mechanisms of optimization of bone regeneration depending on the recipient age under the conditions of using allogeneic bone implants in combination with MSCs and bioactive factors. Methods: biochemical, histological, dual-energy X-ray absorptiometry, enzyme immunoassay, statistical. Results: It has been proven that the use of allografts for plastic surgery of large bone defects that do not heal spontaneously is promising. It has been established that allogeneic MSCs together with an allograft are impractical to use for the reconstruction of bone defects in the case of fresh injuries and without the use of osteoinduction of cells or immunosuppressants. The optimal mode of centrifugation for obtaining a GFBP was determined. It has been proven that the saturation of the allografts with GFBP or their local introduction in 7 days after the operation contributes to the complete replacement of the allograft with bone tissue, regardless of the age of the recipient. Novelty: New scientific knowledge was obtained about the mechanisms of bone regeneration in recipients of different ages under the conditions of reconstruction of large defects with bone allografts along or with GFBP or MSCs. For the first time, the optimal timing of the introduction of GFBP to optimize reparative osteogenesis and accelerate allograft remodeling has been determined. Effectiveness of implementation: the obtained results should be taken into account during the planning of further researchers and the development of treatment tactics for patients with massive bone destruction after high-energy injuries (mine-explosive and gunshot wounds), removal of tumors and major operations on the spine and joints. Scope of use: Medicine.
Product Description
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Ashukina Nataliia Oleksandrivna
Babіch Oleksandr Oleksandrovych
Veliaminova Viktoriia Volodymyrivna
Vorontsov Petro Mykhailovych
Vorontsova Mariia Petrivna
Hrytsenko Anton Volodymyrоvych
Hulida Tetiana Hnativna
Danyshchuk Zynaida Mykolaivna
Katsalap Yelyzaveta Serhiivna
Korzh Mykola Oleksiiovych
Leontieva Fryda Solomonivna
Maltseva Valentyna Yevhenivna
Nikolchenko Olha Anatoliivna
Poshelok Denys Mykhailovych
Samoilova Kateryna Mykhailivna
Sereda Olena Serhiivna
Khmyzov Serhii Oleksandrovych
Yakushkin Yevhen Yuriiovych
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2023-01-27
