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Information × Registration Number 0223U003112, 0122U002297 , R & D reports Title Cellular and molecular genetic mechanisms of salt and drought resistance in barley and prospects for their regulation to improve cereals popup.stage_title Head Blium Yaroslav B., Доктор біологічних наук Registration Date 30-03-2023 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 The method of introduction into culture in vitro, induction of somatic embryogenesis, regeneration and microclonal propagation of plants was developed, and a collection of aseptic plants of different genotypes of wild species of sea barley Hordeum marinum spp was created. Hordeum marinum and Hordeum marinum spp. gussoneanun for further molecular genetic studies to identify the mechanisms of their salt and drought resistance. The CDS sequence of the H.mSCOR potassium channel gene from halophytic barley Hordeum marinum was amplified and cloned. The results of transcriptional profiling of Hordeum marinum plants exposed to salt stress indicate a significant increase in the expression level of this gene, which may indicate an important role of the H.mSCOR potassium channel in the formation of plant resistance to salinity (S.V. Isayenkov). The resulting CDS H.mSCOR plasmid clones are planned to be used for the creation of genetic constructs and further transformation of plants and yeast cells with the aim of elucidating the physiological functions of this channel and creating salt-resistant plant lines. The created clones have a wide range of further applications in the biotechnological improvement of plants or molecular selection.  Product Description popup.authors Yemets Alla I. Ivanova Tetiana S. Isayenkov Stanislav V. Andriyash Ganna S. Bilonozhko Yuliya O. Beiko Natalia Ye. Blume Yaroslav B. Buziashvili Anastasiya Yu. Buy Denis D. Horiunova Inna I. Demchuk Oleh M. Kalafat Liubov O. Karelov Anatoly V. Karpov Pavlo A. Kvasko Anna Yu. Kozub Natalia O. Koliada Oleksandr K. Korkhovyi Vitalii I. Kravets Olena A. Malienko Vadym A. Melnichuk Oleksandr V. Novozhylov Dmytro O. Ozheredov Sergiy P. Pirko Yaroslav V. Pydiura Mykola O. Plohovska Svitlana G. Postovoytova Anastasiya S. Privalikhin Sergiy M. Pushkareva Nadiia О. Raievskyi Oleksii V. Rabokon Anastasiia M. Samofalova Dariya O. Sozinova Oksana I. Spivak Svitlana I. Tigunova Olena O. Khablak Serhii G. Tsyhankov Serhii P. Chugunkova Tetyana V. Shysha Olena M. Shulha Serhii M. popup.nrat_date 2023-03-30 Close