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Information × Registration Number 0210U000412, 0107U000620 , R & D reports Title Morphological, immunohistochemical and molecular biological studies of thyroid papillary carcinomas from children that were born before and after Chernobyl accident. popup.stage_title Head Tronko Mykola Dmytrovich, Bogdanova Tetiana Ivanivna, Registration Date 12-02-2010 Organization State Institution "V.P. Komisarenko Institute of Endocrinology and Metabolism оf the Academy of Medical Scienses of Ukraine" popup.description2 Object: morphological, immunohistochemical and molecular biological changes in thyroid papillary carcinomas of children, born before and after the Chernobyl catastrophe. Aim of the work: determination of structural and molecular genetic features of cancerogenesis in conditions of development of thyroid papillary cancer in children, irradiated as a result of the Chernobyl catastrophe, as compared to papillary carcinomas of children being not exposed to radiation. Methods: morphological, immunohistochemical, molecular biological, statistical. Theoretical and practical results: a comparative pathomorphological analysis of papillary carcinomas of groups of children being identical by age was conducted: born before and after accident, who lived in the most and least contaminated regions by size of tumours, occurrence of capsule and histological structure, and invasion properties, as well as an immunohistochemical analysis of С-cell presence and mcm2 and Ki67 expression. There were no significant differences in between the groups. Papillary carcinomas of solid structure and remote metastases to lungs were more frequent in children from the most contaminated regions, born before the accident. С-cell hyperplasia was established in the non-encapsulated tumours of mainly solid or solid-follicular histological structure around loci of invasive growth. In papillary carcinomas of children, regardless of whether they were exposed to the radioiodine, or born after the accident and being not subject to irradiation in their anamnesis, a higher proliferative potential and higher mitotic activity were observed in the periphery of tumour, that is in areas of invasive growth. A comparative analysis was conducted in relation to RET gene expression in normal and tumour tissue of papillary carcinomas and occurrence of RET/PTC1 and RET/PTC3 rearrangements. The level of RET tyrosine kinase mRNA expression in all of the groups under study was higher in tumour tissue specimens of papillary carcinomas as compared to conditionally normal tissue. RET/PTC3 rearrangements were more frequent in the group of children from the most contaminated regions, born before the Chernobyl accident, that indirectly confirm the role of radiation factor in the pathogenesis of papillary carcinomas. Novelty: a comparative complex analysis of papillary carcinomas in groups of children being identical by age was conducted for the first time: born before and after the accident, who lived in the most and least contaminated regions. The results obtained will be a basis for finding out the mechanisms of radioinduced tumour growth in the future. Efficacy of introducing: the data obtained will be used to improve thyroid tumour diagnostics. Advantage over analogues: it is suggested to analyze the correlation of expression levels of TK and EK domains of RET gene using real-time polymerase chain reaction. Field of application: endocrinology, oncology. Product Description popup.authors Богданова Т. І. Бурко С.В. Воскобойник Л. Г. Зурнаджи Л. Ю. Кашеваров Г. П. Ковзун О.І. Костюченко Н.М. Левчук Н.І. Лукашеня О. С. Негрецький В.А. Плиска Н.П. Пушкарьов В.М. Савчин О.В. Тронько М. Д. Шкаврон Л.В. popup.nrat_date 2020-04-02 Close