Registration Number
0210U003610,
0107U000302
, R & D reports
Title
Сonstruction of producers of recombinant human arginases, and studing effect of preparations of these enzymes on proliferation, apoptosis and autophagy in normal and tumor cells of humans and animals
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Head
Stasyk O.V., Кандидат біологічних наук
Registration Date
12-02-2010
Organization
Institute of cell biology, National Academy of Sciences of Ukraine
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Based on the methylotrophic yeast H. polymorpha designed genetically engineered overproducers of secretory forms of human arginase І and designed a scheme of its purification. Mono- and polyclonal antibodies against recombinant arginase were developed. The inhibition of cell proliferation and induction of apoptosis in tumor cells in vitro during incubation with preparations of recombinant arginase was shown. It was determined that arginine (L-Arg) starvation in vitro leads to the caspase-dependent apoptosis by mitochondrial type in sensitive to the deficiency of L-Arg cells of human tumors (hepatocellular carcinoma and melanoma). It is shown that the ability of transformed cells to resynthesis L-Arg, production of nitric oxide and polyamines are not defining in the response of these tumors to the lack of L-Arg. Revealed that the processes of recycling of intracellular proteins, autophagy and proteasome degradation play a protective role in conditions of stress caused by starvation for arginine.
Product Description
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Ігуменцева Н.І.
Барська М.Л.
Бобак Я.П.
Винницька Б.О.
Вовк О.І.
Воробець З.Д.,
Дашко С.В.
Захарів О.Я.
Красовська О.С.
Курліщук Ю.В.
Линів Л.С.
Маєвська О.М.
Ржепецький Ю.А.
Сибірна Н.О.
Стасик О.Г.
Чень О.І.
Чернишук С.В.
Шуваєва Г.Ю.
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2020-04-02
