Registration Number
0211U001404,
0107U011259
, R & D reports
Title
To substantiate and develop the alternative conception of embryos reconctruction and directions of molecular-genetic investigations for using in farm animal reproduction
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Head
Sushko Aleksey Borisovich,
Registration Date
02-02-2011
Organization
Ternopil institute of agroindustrial production of the National academy of agrarian sciences of Ukraine
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The chimera embryos of mouse from 2-5 by agregation of whole embryos with the use as containers for agregation of empty ZP of mouse and cow, and also glass rings are got. It is got chimera agregated embryos from part of cells of whole frozen-thawed embryos of mouse, incorporated together in the cavity of ZP of cow. Trofoblastic bubbles are got by the ICM cutting off with part of trofoblast adjoining to her from ordinary and chimera to the blastotsyst mouse. The videotape recordings of changes of form of the ZP of cattle cut on a half-round are got in two perpendicular planes at introduction to their cavity of microtools and the scopes of change of their parameters are certain during manipulations. Oocytes is worked on rabbit and horse and the method of manual delete of chromosomes from oocytes mammals is developed and patented. The complete set of microtools "Guillotine", and also technique of manipulation is developed by him on the separation from oocytes PPLCh and on the ICM separation from blastotcyst for the TBV receipt. Fusion of cells of trofoblastt in the area of contact of blastotscst is attained, agglutinated in a pair by FGA, as a result of the EIF action. At the same terms development is got to the stage of extended blastocyst and chimera embryo from two agglutinated in the pair of oocytes, washed-out from the oviducts of mouse together with 8-cellular embryos in 73-75 hours after the injection of hHG. The method of stimulation of the EIF maturation of oocytes mammals out of organism and method of the automatic determination of efficiency of the OCC stimulation is developed. The state of mature is explored on morphological indexes conductivity oocytes, got at stimulation of maturation EIF or PMGS and hHG and in the natural sexual cycle. It is shown that exactly the PMGS influencing at superovulation results in the selection in the bullet of the oocytes second group which is characterized by the promoted conductivity. The method of determination of follicle phase of estral cycle of cows is developed, when temperature difference in the cavity of vagina on two certain distances from vulvum cows and heifers are not exceeded by 0,08оС. Methodical recommendations after determination of phases of spontaneous sexual cycle of cows on the morphofunctional traits of ovaries are developed.
Product Description
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Камишан Наталя Василівна
Колеснікова Алла Олександрівна
Лісін Вадим Іванович
Лісіна Катерина Генадіївна
Медведовська Марина Ігорівна
Помітун Лариса Іванівна
Саминіна Марина Генадіївна
Сербін Максим Євгенович
Ткаченко Олена Юріївна
Чернецов Олег Олександрович
Шахова Юлія Юріївна
Шигимага Віктор Олександрович
Шкавро Наталя Миколаївна
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2020-04-02
