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Information × Registration Number 0212U009017, 0112U008186 , R & D reports Title Role of adaptor prorein Ruk/CIN85 in the development of human nervous system pathological. popup.stage_title Head Byts N.V., Registration Date 26-12-2012 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 As a result of this work stable transfected clonal populations of rat pheochromocytoma cell line PC12 with overexpression of Ruk/CIN85 associated with the green fluorescent protein GFP were obtained. Its expression level was analyzed by Western blot and confocal microscopy. It was found that PC12/GFP-Ruk/CIN85 cells were characterized by the ability to form clusters and to clone growth. The ability to differentiate by neuronal type was detected to be completely blocked in PC12/GFP-Ruk/CIN85 cells, whereas the wild-type cells formed spines in a medium with low serum content after NGF addition, which were similar to the neurons of the sympathetic nervous system. In addition, overexpression of Ruk/CIN85 led to constitutive activation of proteinkinase B (Akt) and increased activation of ERK1/2 after NGF action. A high expression level of Id1 and low expression level of MATH2/NEUROD6 were found in EGFP-Ruk-overexpressing cells compared to control cells. Product Description popup.authors Биць Надія Вікторівна Пасічник Ганна Володимирівна Поворознюк Ольга Олександрівна popup.nrat_date 2020-04-02 Close