Registration Number
0212U009017,
0112U008186
, R & D reports
Title
Role of adaptor prorein Ruk/CIN85 in the development of human nervous system pathological.
popup.stage_title
Head
Byts N.V.,
Registration Date
26-12-2012
Organization
A.V.Palladin Institute of Biochemistry of National Academy of Sciences
popup.description2
As a result of this work stable transfected clonal populations of rat pheochromocytoma cell line PC12 with overexpression of Ruk/CIN85 associated with the green fluorescent protein GFP were obtained. Its expression level was analyzed by Western blot and confocal microscopy. It was found that PC12/GFP-Ruk/CIN85 cells were characterized by the ability to form clusters and to clone growth. The ability to differentiate by neuronal type was detected to be completely blocked in PC12/GFP-Ruk/CIN85 cells, whereas the wild-type cells formed spines in a medium with low serum content after NGF addition, which were similar to the neurons of the sympathetic nervous system. In addition, overexpression of Ruk/CIN85 led to constitutive activation of proteinkinase B (Akt) and increased activation of ERK1/2 after NGF action. A high expression level of Id1 and low expression level of MATH2/NEUROD6 were found in EGFP-Ruk-overexpressing cells compared to control cells.
Product Description
popup.authors
Биць Надія Вікторівна
Пасічник Ганна Володимирівна
Поворознюк Ольга Олександрівна
popup.nrat_date
2020-04-02
