Registration Number
0213U006405,
0108U011159
, R & D reports
Title
Research of apoptosis mechanisms of immunecompetent cells at pathoses on a plain and in a highland
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Head
Bagdasarova Ingretta. Tarady Nelli,
Registration Date
17-05-2013
Organization
International Center Astronomical, Medical and Ecological Research
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Research object: 114 patients (75 children in age from 5 to 12 with the immune- inflammatory diseases of kidneys, 39 children with a chronic and acute infectious-inflammatory disease - mononuclejsis), conditionally healthy habitants of highland (21) and plain (19), conditionally healthy children in age from 5 to 12 (17). Research material: venous blood, micro biopsy material of kidneys, preparations of thymus and spleen of rats. Aim of work: to study the role of apoptosis and differential markers of ICC in chronization of inflammatory processes in the conditions of plain and highland. Research methods: biochemical, clinical-laboratorial, immunofluorescence, microbiological, instrumental, US. The expression, distribution, colocalization of apoptosis markers Fas/ CD95+, Bax, receptors to TNF? and IFN?, Bcl - 2, IL - 2R, iNOs, transmembrane IgA, IgM, IgG, IgD in CD4, CD7, CD8, CD16, CD20, CD57, CD25 have been investigated in ICC and biopsy material of kidneys, spleens and thymus of rats. Markers were exposed by means of the monoclonal serums, marked by fluorescence marks. Results took into account by means of multimedia luminescent (Carl Zeizz - 250SF, computer program Multichenel AxioVision 4.4) and confocal laser and electronic scanning microscopy (Axioskop - 2 LSM 5 PASCAL Carl ZEISS with helium-neon and an argon lasers) at the one moment three- and four-coloured painting. The images were processed by means of the computer program LSV510. For the habitants of highland a basic proapoptosis factor with the autocrine and paracrine mechanism of action was NO. Thus Fas-dependent apoptosis is depressed and the decline of Annexin V expression is marked, that can promote to chronization of inflammatory diseases in a highland due to the decline of elimination, antiinflammatory and anticoagulating activity of the immune system. A chronic immune-inflammatory process is characterized by activation of receptor-depended apoptosis on TNF?-way with activation simultaneously of receptors to INF?, hyperexpression of iNOs and sluggishness of Fas-receptor apoptosis. The expression of antiapoptosis protein Вcl2 is activated in cytotoxic T-cell at the patients with chronic glomerulonephritis. As a result of treatment expression of TNF? increases considerably and Fas- receptor apoptosis activates. The quality and quantitative changes of colocalization and distributions of expressed markers of apoptosis in ICC and cross-correlation close intercommunications between markers after the course of pathogenetic therapy are defined. Comparative research of the markers of apoptosis at mononucleosis has shown the high degree of their expression, more considerably expressed for patients with acute EB virus infection. All studied markers of apoptosis exceeded control values in 2-3 and more than time. Both at acute and chronic processes of EB virus infection the level of Bcl-2 expression of were statistically equivalent. Activating of apoptosis at infectious-inflammatory process flows on a receptor-depended way through receptors for Fas, INF? and TNF?. AnnV expression in ICC of chronic patients was higher, than at acute process. Experimental researches for rats in the conditions of influence of acute and chronic hypoxia showed that structural alterations, mitochondrial disfunctions are closely related to duration and degree of adaptation to the hypoxia and the processes of apoptosis at these terms flow on mitochondrial and oxide nitrogen-activated way. Individual map membrane and differential markers expressed on ICC on a background the conducted immunecorrect treatment, is the point of counting out for the correction of immune disorders on the stages of pathogenetic therapy. KEYWORDS: immunecompetent cell (ICC), expression of receptors, markers of apoptosis and differentiation, chronic immune-inflammatory and infectiously-inflammatory processes, glomerulonephritis, mononucleosis - EB virus infection, hypoxia, extremely-high frequencies (EHF) therapy.5481548154815481
Product Description
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2020-04-02
