Registration Number
0214U008784,
0111U000518
, R & D reports
Title
Fundamentals of technology a new putative antitumour cytokine EMAP II and its mutant forms with increased antiangiogenic effect
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Head
Kornelyuk Alexander Ivanovich,
Registration Date
16-10-2014
Organization
Institute of Molecular Biology and Genetics
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The study of the interaction of cytokine EMAP II with beta-cyclodextrin. In model experiments by reacting L-tryptophan with beta-cyclodextrin increase of fluorescence detected Trp, which may indicate its binding with the interior of the beta-cyclodextrin. For recombinant cytokine EMAP IIf found that nevidschepleny N-terminal fragment (42 a.a.) Blocks a local conformational change - the output Trp residue onto the surface of the protein. With increasing temperature up to 60 degrees Celsius observed weak binding of beta-cyclodextrin as a result of screening residue Trp. In the study of EMAP IIe with proteolysis fragment 42 a.a. there is a local conformational transition associated with the exposure of Trp residue on the protein surface. For complex EMAP IIe with beta-cyclodextrin as the temperature increases observed blocking local conformational transition and stabilization of the protein globule EMAP IIe in the complex. For complex EMAP IIe with HP-beta-cyclodextrin at elevated temperatures also found blocking local conformational transition and stabilization of the protein globule EMAP IIe in the complex. According to computer modeling can conclude provided interaction implemented between beta-cyclodextrin molecule and the protein EMAP II, characterized by bonding molecules of an oligosaccharide with a nonpolar amino acid residues - proline and glycine and polar positively charged amino acid residues.
Product Description
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Козлов Олександр Вадимович
Кордиш Марія Олександрівна
Ложко Дмитро Миколайович
Резніков Олександр Григорович
Чайковська Людмила В'ячеславівна
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2020-04-02
