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Registration Number

0215U006250, 0112U002334 , R & D reports

Title

Polymorphism of stress genes and molecular biochemical mechanisms of cell protection in plants

Head

Roman A. Volkov, Доктор біологічних наук

Registration Date

30-01-2015

Organization

Yuri Fedkovych Chernivtsi National University

Description

The level of oxidative damage of proteins and lipids, amount of stress-associated metabolites (ascorbate, glutathione), activities and isoenzyme patterns of catalase, ascorbate peroxidase (APX), guaiacol peroxidase (POD), glutathione reductase (GR) and dehydroascorbate reductase (DHAR) were determined. Also, the changes of expression levels of the Apx, Cat and sHsp genes under elevated temperatures and under stress caused by increased Cu2+ and Cd2+ concentrations were studied in wild-type plants and CAT2 and CAT3 knock-out mutants. A transgenic line with increased catalase activity was created and analyzed. It was demonstrated that enzymatic and non-enzymatic oxidative stress-resistance mechanisms, which are aimed to compensate the deficiency of catalase isoforms, are activated in mutants. A suppression of heat-shock gene transcription and accelerated secondary response could be evidenced for the first time.

Product Description

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Буздуга Інна Миколаївна
Волков Роман Анатолійович
Діденко Наталія Олександрівна
Касіянчук Руслана Михайлівна
Панчук Ірина Ігорівна
Руснак Тетяна Олександрівна
Тинкевич Юрій Олегович
Череватов Олександр Володимирович
Язловицька Людмила Степанівна