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Registration Number

0216U000032, 0114U001096 , R & D reports

Title

Study of polimicrobial associations of biotops and immune violations at patients with the diagnosed infectiously-inflammatory complications at myeloid origin leukemia

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Head

A.P. Rybalska,

Registration Date

15-01-2016

Organization

Kiev Research Institute of Haematolojy and Blood Transfusion

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Objects. There were 38 patients, among them: 24 patients with AML, 2 - with MDS, 12 - with CML; 137 strains of microorganisms. Aim: to determine the ability of biofilm formation and adhesion properties of microorganisms - causative agents of infectious and inflammatory processes and to clarify their relationship with the severity of the course of complications. Methods: laboratory-clinical, haematological, immunohistochemical, microbiological, immunological, serological. Results: From the upper airways 29 patients with AML and CML Izu isolated 137 strains of microorganisms, including pathogens by sepsis, pneumonia, chronic bronchitis and mikoznoho lesions of the oral cavity and pharynx, bacteria genera Staphylococcus, Enterococcus, Micrococcus and Klebsiella, Candida glabrata, C. krusei and Candida sp .. To probably factors IIC strains were identified genera Staphylococcus and Enterococcus, Klebsiella pneumoniae, Moraxella catarrhalis, yeast and filamentous fungi - Candida sp. and Aspergillus sp., that are isolated in the diagnostically significant titers. The sensitivity of existing and probable pathogens IIC to 46 antibiotic and to 6 antimycotics was determined. At 52.6% serum from 19 patients with AML and CML was revealed antifungal antibodies in titers of 1: 1600 to 1: 12800. Was defined the contents of anti-Aspergillus antibodies in serum 19 patients with AML and HML, among which in the 57.9% of persons the value of marker of aspergillus (K) fluctuated within the "second cut-off" (1,1-4,9). Established that clinical strains of microorganisms genus of Staphylococcus, characterized by the presence of pathogenic properties in the (57,1-100,0)% of cases. Of the 14 clinical strains of staphylococci highly adhesive were 42.86%. Of the 19 clinical cultures of staphylococci 86.4% were characterized by a high ability to formation of biofilm, 13.6% - average. 75% of strains of Candida actively formed biofilm, 25% had an average degree of this features. In patients of AML with IIC it was determined increase indicators CD-4, CD-8, CD-16 lymphocyte on the background of ineffective of the final phase of phagocytosis of neutrophils. In patients of AML and CML the increasing levels of CRP up to 16-384 mg/l it was determined. High level of formation of biofilm and adhesiveness of microorganisms the causing persistence of pathogens infectious-inflammatory complications. This, on the background of violations of innate and adaptive immunity in patients with myeloid neoplasia, leads to the development of local or generalized inflammation processes.Medical and social effect. Medicine.

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Кисельова О.А.
Мельник О.А.
Немировська Л.М.
Перехрестенко Т.П.
Скачкова Н.К.
Третяк Н.М.

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2020-04-02